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	A novel biosensor for the rapid determination of biochemical oxygen demand
	Chen, Jin-Song ; Zhang, Li-Sheng ; Wang, Jian-Long
	2010-05-10 ; 2010-05-10
关键词	BOD BOD biosensor wastewater water quality water pollution SENSOR BOD Environmental Sciences Public, Environmental & Occupational Health
中文摘要	Objective To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand (BOD) which is developed by our research group based on suspended inumobilized microbial cell system in a completely mixed determining chamber as a substitute of the traditional membrane system. Methods Activated sludge was immobilized by PVA gel and used as a bio-sensing element. The novel biosensor was used to measure the short time BOD value and the conventional cultivation method was used for BOD5 measurement. Results A linear relationship was observed for the difference between the current and the concentration of glucose-glutamic acid (GGA) solution below 200 mg/L with a correlation coefficient of 0.995. The optimal response of the sensor was obtained at pH 7.0 and 30 degrees C. The sensor response was within 15 min and was reproducible within +/- 5% of the mean in a series of eight samples containing 75 mg/L BOD using standard GGA solution. The novel sensor response was found to be fairly constant over a period of 30 days, with 5% fluctuations. Conclusion A relatively good agreement is found between BOD estimated by the novel BOD biosensor and that determined by the conventional 5-day BOD method. This novel BOD biosensor has good sensitivity, stability and reproducibility.
语种	英语 ; 英语
出版者	CHINESE ACAD PREVENTIVE MEDICINE ; ORLANDO ; C/O ACADEMIC PRESS INC, 6277 SEA HARBOR DR, ORLANDO, FL 32887-4900 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/22724]
专题	清华大学
推荐引用方式 GB/T 7714	Chen, Jin-Song,Zhang, Li-Sheng,Wang, Jian-Long. A novel biosensor for the rapid determination of biochemical oxygen demand[J],2010, 2010.
APA	Chen, Jin-Song,Zhang, Li-Sheng,&Wang, Jian-Long.(2010).A novel biosensor for the rapid determination of biochemical oxygen demand..
MLA	Chen, Jin-Song,et al."A novel biosensor for the rapid determination of biochemical oxygen demand".(2010).

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