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	Regulation of charged groups and laminin patterns for selective neuronal adhesion
	Liu, B. F. ; Ma, J. ; Xu, Q. Y. ; Cui, F. Z.
	2010-05-10 ; 2010-05-10
关键词	cell adhesion hippocampal neuron laminin micro-contact printing poly(ethylenimine) protein patterning SOFT LITHOGRAPHY NEURITE OUTGROWTH POINT CONTACTS CELL-CULTURE GROWTH GUIDANCE RAT NETWORKS SURFACES BIOMATERIALS Biophysics Chemistry, Physical Materials Science, Biomaterials
中文摘要	Primary neuronal cultures on substrates patterned with extracellular matrix proteins such as laminin have yielded much information regarding the physiological characteristics of neuronal cells in vitro. Surface charge also influences neuronal adherence, and a positive charge can have stimulatory effects. The attraction between laminin patterns and polycation films are of interest in the study of neuronal adhesion. We cultured primary hippocampal neurons on poly(ethylenimine) (PEI) films with laminin grids and evaluated their viability and morphology by means of fluorescent microscopy after 5-7 days. The results showed that the neurons did not form networks on the laminin grids. It is inferred that the PEI films were more favourable for neuronal adhesion than the laminin grid. (c) 2006 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/21750]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, B. F.,Ma, J.,Xu, Q. Y.,et al. Regulation of charged groups and laminin patterns for selective neuronal adhesion[J],2010, 2010.
APA	Liu, B. F.,Ma, J.,Xu, Q. Y.,&Cui, F. Z..(2010).Regulation of charged groups and laminin patterns for selective neuronal adhesion..
MLA	Liu, B. F.,et al."Regulation of charged groups and laminin patterns for selective neuronal adhesion".(2010).

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