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	Human neutrophils reaction to the biodegraded nano-hydroxyapatite/collagen and nano-hydroxyapatite/collagen/poly(L-lactic acid) composites
	Liao, S ; Tamura, K ; Zhu, YH ; Wang, W ; Uo, M ; Akasaka, T ; Cui, FZ ; Watari, F
	2010-05-10 ; 2010-05-10
关键词	neutrophils nano-hydroxyapatite biodegraded COLLAGEN-BASED COMPOSITE MINERALIZED COLLAGEN IN-VITRO MATRIX DEGRADATION EXPRESSION TITANIUM SCAFFOLD APATITE NICKEL Engineering, Biomedical Materials Science, Biomaterials
中文摘要	The impact of biodegraded nano-hydroxyapatite/collagen (nHAC) composite and nano-hydroxyapatite/ collagen/poly(L-lactic acid) (nHAC/PLA) scaffold composite on neutrophils reaction was evaluated in vitro. Neutrophils were separated from human peripheral blood of healthy subjects. The nHAC and nHAC/PLA materials were immersed in the D-Hanks' Balanced Salt Solution (DHBSS) for I day, 7 days and 2, 4, 8 weeks (37 degrees C) as testing solution, which mixed with the neutrophils for 1 h. Both of the nHAC and nHAC/PLA materials were shown the same cell Survival rate as blank control, but the lactate dehydrogenase (LDH) and tumor necrosis factor alpha (TNF-alpha) released from the neutrophils were increased significantly after the 2 weeks in nHAC sample. The possible reason relied on the high concentration of calcium due to the quick biodegradation of the nHAC material. Before 2 weeks, the LDH value of nHAC/PLA is higher than that of nHAC sample that corresponded to the initial PLA degradation in vitro. This study provided the biocompatibility test of neutrophils other than common methods, such as osteoblastic cells for biomimetic materials. Moreover, it demonstrated the calcium concentration stimulating effect for cytokine release from neutrophils. (c) 2005 Wiley Periodicals, Inc.
语种	英语 ; 英语
出版者	WILEY-LISS ; HOBOKEN ; DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/21336]
专题	清华大学
推荐引用方式 GB/T 7714	Liao, S,Tamura, K,Zhu, YH,et al. Human neutrophils reaction to the biodegraded nano-hydroxyapatite/collagen and nano-hydroxyapatite/collagen/poly(L-lactic acid) composites[J],2010, 2010.
APA	Liao, S.,Tamura, K.,Zhu, YH.,Wang, W.,Uo, M.,...&Watari, F.(2010).Human neutrophils reaction to the biodegraded nano-hydroxyapatite/collagen and nano-hydroxyapatite/collagen/poly(L-lactic acid) composites..
MLA	Liao, S,et al."Human neutrophils reaction to the biodegraded nano-hydroxyapatite/collagen and nano-hydroxyapatite/collagen/poly(L-lactic acid) composites".(2010).

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