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	The impact of PHB accumulation on L-glutamate production by recombinant Corynebacterium glutamicum
	Liu, Qian ; Ouyang, Shao-Ping ; Kim, Jonghyok ; Chen, Guo-Qiang
	2010-05-10 ; 2010-05-10
会议名称	JOURNAL OF BIOTECHNOLOGY ; 10th International Symposium on Biological Polyesters ; Minneapolis, MN ; Web of Science
关键词	corynebacterium glutamicum L-glutamate PHB polyhydroxybutyrate lactate ESCHERICHIA-COLI ACID PRODUCTION CORYNEFORM BACTERIA GLUCOSE GENE POLYHYDROXYALKANOATES POLYHYDROXYBUTYRATE EXPRESSION SYNTHASE PYRUVATE Biotechnology & Applied Microbiology
中文摘要	Corynebacterium glutamicum, a gram-positive soil bacterium, has been used extensively for the industrial production of L-glutamate and other amino acids. In this study, an Escherichia coli-C. glutamicum shuttle expression plasmid harboring polyhydroxybutyrate (PHB) synthesis genes, phbCAB from Ralstonia eutropha, was constructed under the Ptrc promoter. C glutamicum harboring this plasmid accumulated 3-13% PHB with a weight average molecular mass of 125,400 and a polydispersity of 11.3 when grown on glucose. PHB synthesis related enzyme activities including beta-ketothiolase (PhbA), acetoacetyl-CoA reductase (PhbB) and PHB synthase (PhbC) were found to be constitutively produced independent of IPTG. L-Glutamate production increased 39-68% in two C. glutamicum strains harboring PHB synthesis genes compared with their parent strains in shake flask experiments. In fermentor studies, the recombinant produced approximately 23% more L-glutamate compared with that of the wild type, and yielded less intermediate metabolites or by-products including alpha-ketoglutarate, L-glutamine and lactate. These results suggested that the expression of phbCAB genes in C. glutamicum could help regulate glutamate production metabolism. This demonstrated that the expression of PHB synthesis genes has a positive effect on L-glutamate production in C. glutamicum. (c) 2007 Elsevier B.V. All rights reserved.; Univ Minnesota
会议录出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/20012]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, Qian,Ouyang, Shao-Ping,Kim, Jonghyok,et al. The impact of PHB accumulation on L-glutamate production by recombinant Corynebacterium glutamicum[C]. 见:JOURNAL OF BIOTECHNOLOGY, 10th International Symposium on Biological Polyesters, Minneapolis, MN, Web of Science.

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