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	The pharmacokinetics analysis of the phosphoryl peptides in MCF-7/ADR cells
	Zhang, Peng ; Liu, Mian ; Liu, Hongxia ; Xie, Zhenhua ; Liu, Feng ; Cai, Zongwei ; Jiang, Yuyang
	2010-05-10 ; 2010-05-10
会议名称	PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS ; 17th International Conference on Phosphorus Chemistry ; Xiamen, PEOPLES R CHINA ; Web of Science
关键词	determination high-performance liquid chromatography MCF-7/ADR cells N-diisopropyloxyphosphoryl-valine-phenylaianine-3,4,5-trimethoxyaniline AMINO-ACIDS LIQUID-CHROMATOGRAPHY INHIBITORS SEPARATION Chemistry, Inorganic & Nuclear
中文摘要	We have developed and validated a practical high-performance liquid chromatography method to determine the concentration of N-diisopropyloxyphosphoryl-valine-phenylalanine-3, 4, 5-trimethoxy aniline (DIPP-Val-Phe-TMOA) in Adriamycin-resistant human breast adenocarcinoma (MCF-7/ADR) cells. First, DIPP-Val-phe-TMOA was extracted from MCF-7 cells with perchloric acid, followed by a cleanup procedure with centrifugal separation, and then determinated by HPLC using a Waters C18 column with a gradient elation (solvent A: 10 mM ammonium acetate at pH 7.0, B: acetonitrile). The flow rate was maintained at 1.0 ml/min at room temperature. The detection wavelength was at 254 nm. Meanwhile, this validated method was successfully applied to analyze the concentration levels and the pharmacokinetics parameters of DIPP-Val-Phe-TMOA in MCF-7/ADR cells.
会议录出版者	TAYLOR & FRANCIS LTD ; ABINGDON ; 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/19978]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Peng,Liu, Mian,Liu, Hongxia,et al. The pharmacokinetics analysis of the phosphoryl peptides in MCF-7/ADR cells[C]. 见:PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS, 17th International Conference on Phosphorus Chemistry, Xiamen, PEOPLES R CHINA, Web of Science.

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