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	Two-photon fluorescence anisotropy imaging - art. no. 60472R
	Li Wei ; Wang Yi ; Shao Hanrong ; He Yonghong ; Ma Hui
	2010-05-10 ; 2010-05-10
会议名称	Fourth International Conference on Photonics and Imaging in Biology and Medicine, Pts 1 and 2 ; 4th International Conference on Photonics and Imaging in Biology and Medicine ; Tianjin, PEOPLES R CHINA ; Web of Science
关键词	fluorescence anisotropy two-photon excitation tumor cell membrane fluidity berberine tumor cell MICROSCOPY CELLS LASER Biophysics Optics Physics, Applied Imaging Science & Photographic Technology Spectroscopy
中文摘要	We have developed a novel method for imaging the fluorescence intensity and anisotropy by two-photon fluorescence microscopy and tested its capability in biological application. This method is applied to model sample including FITC and FITC-CD44 antibody solution and also FITC-CD44 stained cells. The fluorescence anisotropy (FA) of FITC-CD44ab solution is higher than the FITC solution with the same concentration. The fluorescence in cell sample has even higher FA than in solution because the rotation diffusion is restrained in membrane. The method is employed to study the effect of berberine a kind of Chinese medicine, on tumor metastasis. The results indicated that tumor cell membrane fluidity is decreasing with increasing the concentration of berberine in culture medium.
会议录出版者	SPIE-INT SOC OPTICAL ENGINEERING ; BELLINGHAM ; 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/19964]
专题	清华大学
推荐引用方式 GB/T 7714	Li Wei,Wang Yi,Shao Hanrong,et al. Two-photon fluorescence anisotropy imaging - art. no. 60472R[C]. 见:Fourth International Conference on Photonics and Imaging in Biology and Medicine, Pts 1 and 2, 4th International Conference on Photonics and Imaging in Biology and Medicine, Tianjin, PEOPLES R CHINA, Web of Science.

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