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	Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe
	Hu, ZH ; Wang, J ; Liang, JW
	2010-05-10 ; 2010-05-10
会议名称	OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS: DIAGNOSTICS AND TREATMENT II , PTS 1 AND 2 ; Conference on Optics in Health Care and Biomedical Optics ; Beijing, PEOPLES R CHINA ; Web of Science ; INSPEC
关键词	optical manipulation biological cell manipulation fiber trapping optical force force measurement DIELECTRIC PARTICLES MANIPULATION ARRAY Medical Laboratory Technology Optics Radiology, Nuclear Medicine & Medical Imaging
中文摘要	In conventional optical tweezers system a high numerical aperture (NA) objective is employed both to image and to generate a gradient force toward the focus on sample particles, so the system is complex and expensive especially for the multi-optical-tweezers system. We built a novel simple optical trapping system based on a lensed optical fiber probe. This new method offers several other advantages over the conventional optical tweezers. The trapping system we built includes a laser coupling unit, a multi-dimensional probe manipulating unit, a sample nano-positioning unit, and a microscopy imaging unit. Based on the system, a yeast cell is trapped and manipulated on the chamber bottom by the lensed fiber probe, and the optical trapping forces acting on the yeast cell as a function of the offset are measured and discussed in different directions by the static method and the dynamic one respectively with various powers. The results by the two measurement methods coincide with each other, and the detail experimental procedure and the data processing of the two methods are introduced in this paper.
会议录出版者	SPIE-INT SOC OPTICAL ENGINEERING ; BELLINGHAM ; 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/19242]
专题	清华大学
推荐引用方式 GB/T 7714	Hu, ZH,Wang, J,Liang, JW. Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe[C]. 见:OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS: DIAGNOSTICS AND TREATMENT II , PTS 1 AND 2, Conference on Optics in Health Care and Biomedical Optics, Beijing, PEOPLES R CHINA, Web of Science, INSPEC.

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