Protein refolding assisted by periodic mesoporous organosilicas | |
Wang, Xiqing ; Lu, Diannan ; Austin, Rebecca ; Agarwal, Arun ; Mueller, Leonard J. ; Liu, Zheng ; Wu, Jianzhong ; Feng, Pingyun | |
2010-05-07 ; 2010-05-07 | |
关键词 | RESPONSIVE CONTROLLED-RELEASE MOLECULAR-SIEVES BIOACTIVE GLASSES ESCHERICHIA-COLI INCLUSION-BODIES DELIVERY-SYSTEM ORGANIC GROUPS SILICA LYSOZYME RENATURATION Chemistry, Physical |
中文摘要 | Herein we report a new strategy for protein refolding by taking advantage of the unique surface and pore characteristics of ethylene-bridged periodic mesoporous organosilica (PMO), which can effectively entrap unfolded proteins and assist refolding by controlled release into the refolding buffer. Hen egg white lysozyme was used as a model protein to demonstrate the new method of protein refolding. Through loading of denatured proteins inside uniform mesoporous channels tailored to accommodate individual protein, protein aggregation was minimized, and the folding rate was increased. Poly(ethyleneglycol) (PEG)-triggered continuous release of entrapped denatured lysozyme allowed high-yield refolding with high cumulative protein concentrations. The new method enhances the oxidative refolding of lysozyme (e.g., over 80% refolding yield at about 0.6 mg/mL). |
语种 | 英语 ; 英语 |
出版者 | AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/17730] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Wang, Xiqing,Lu, Diannan,Austin, Rebecca,et al. Protein refolding assisted by periodic mesoporous organosilicas[J],2010, 2010. |
APA | Wang, Xiqing.,Lu, Diannan.,Austin, Rebecca.,Agarwal, Arun.,Mueller, Leonard J..,...&Feng, Pingyun.(2010).Protein refolding assisted by periodic mesoporous organosilicas.. |
MLA | Wang, Xiqing,et al."Protein refolding assisted by periodic mesoporous organosilicas".(2010). |
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