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	Dynamic control of protein folding pathway with a polymer of tunable Hydrophobicity
	Lu, Diannan ; Wu, Jianzhong ; Liu, Zheng
	2010-05-07 ; 2010-05-07
关键词	CARBONIC-ANHYDRASE-B ARTIFICIAL CHAPERONE MOLECULAR SIMULATION RECOMBINANT PROTEINS RESPONSIVE POLYMER ESCHERICHIA-COLI INCLUSION-BODIES AGGREGATION MODEL RENATURATION Chemistry, Physical
中文摘要	While the knowledge of protein folding in a dilute solution is now well-advanced, little is known of the influence of surrounding conditions on the folding kinetics, in particular when the protein is in a dynamically responsive environment. Here we report a new procedure to control the pathways of protein folding by using a thermally responsive polymer that varies its hydrophobicity concomitant with the protein structural changes. The advantages of folding in a dynamic environment have been demonstrated first by Langevin dynamics simulations on the basis of coarse-grained models for both the protein and polymer and then by experiments for lysozyme refolding in the presence of poly(N-isopropylacrylamide-co-N-tert-butylacrylamide), a thermal responsive polymer that varies its hydrophobicity in response to temperature. The simulation suggests that decreasing the polymer hydrophobicity during the folding process may result in an optimized free-energy landscape that enhances both the folding yield and kinetics. The experiments affirm that an optimal folding condition can be identified when structural transitions of the protein collaborate with the polymer hydrophobicity tuned by variation of temperature.
语种	英语 ; 英语
出版者	AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17713]
专题	清华大学
推荐引用方式 GB/T 7714	Lu, Diannan,Wu, Jianzhong,Liu, Zheng. Dynamic control of protein folding pathway with a polymer of tunable Hydrophobicity[J],2010, 2010.
APA	Lu, Diannan,Wu, Jianzhong,&Liu, Zheng.(2010).Dynamic control of protein folding pathway with a polymer of tunable Hydrophobicity..
MLA	Lu, Diannan,et al."Dynamic control of protein folding pathway with a polymer of tunable Hydrophobicity".(2010).

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