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	Effect of several factors on soluble lipase-mediated biodiesel preparation in the biphasic aqueous-oil systems
	Chen, Xin ; Du, Wei ; Liu, Dehua
	2010-05-07 ; 2010-05-07 ; OCT
关键词	soluble lipase oleic acid esterification biodiesel CANDIDA-ANTARCTICA LIPASE SOLVENT-FREE MEDIUM SUNFLOWER OIL CATALYZED TRANSESTERIFICATION IMMOBILIZED LIPASE ORGANIC-SOLVENT FUEL PRODUCTION VEGETABLE-OIL SOYBEAN OIL ALCOHOLYSIS Biotechnology & Applied Microbiology
中文摘要	Biodiesel is increasingly perceived as an important component of solutions to the important current issues of fossil fuel shortages and environmental pollution. Utilization of soluble lipase offers an alternative approach to lipase-catalyzed biodiesel production using immobilized enzyme or whole-cell catalysis. Soluble lipase NS81020, produced by submerged fermentation of genetically modified Aspergillus oryzae microorganism, was first proposed here as the catalyst of biodiesel preparation with oleic acid in the biphasic aqueous-oil systems. The effect factors such as enzyme concentration, water content, temperature, molar ratio of methanol to oil, stirring rate and pH of buffer solution on the esterification rate were investigated systematically. The reaction time could be shortened with the increasing of enzyme concentration as long as the maximum enzyme absorptive capacity on the interface in the biphasic aqueous-oil systems was not achieved. The optimal water content in the biphasic aqueous-oil systems was 10 wt% by oleic acid weight. The reaction rate was enhanced with the increasing molar ratio of methanol to oil, the increasing stirring rate or the decreasing temperature. Although soluble lipase NS81020 had lower activity at pH 10.55, hydroxyl ion conduced to restrain hydrolysis of methyl ester and facilitated the reaction toward the methyl ester formation.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17681]
专题	清华大学
推荐引用方式 GB/T 7714	Chen, Xin,Du, Wei,Liu, Dehua. Effect of several factors on soluble lipase-mediated biodiesel preparation in the biphasic aqueous-oil systems[J],2010, 2010, OCT.
APA	Chen, Xin,Du, Wei,&Liu, Dehua.(2010).Effect of several factors on soluble lipase-mediated biodiesel preparation in the biphasic aqueous-oil systems..
MLA	Chen, Xin,et al."Effect of several factors on soluble lipase-mediated biodiesel preparation in the biphasic aqueous-oil systems".(2010).

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