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	3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae
	Hao, Jian ; Lin, Rihui ; Zheng, Zongming ; Sun, Yan ; Liu, Dehua
	2010-05-07 ; 2010-05-07
关键词	3-Hydroxypropionaldehyde Klebsiella pneumoniae 1 3-Propanediol Feeding strategy CLOSTRIDIUM-BUTYRICUM DSM-5431 ANAEROBIC CONTINUOUS-CULTURE MICROBIAL-PRODUCTION CITROBACTER-FREUNDII FERMENTATION DISSIMILATION ACCUMULATION METABOLISM MUTANTS ENZYMES Biotechnology & Applied Microbiology
中文摘要	3-Hydroxypropionaldehyde (3-HPA) is a toxic intermediary metabolite in the biological route of 1,3-propanediol biosynthesis from glycerol. 3-HPA accumulated in culture medium would arouse an irreversible cessation of the fermentation process. The role of substrate (glycerol) on 3-HPA accumulation in aerobic fermentation was investigated in this paper. 1,3-Propanediol oxidoreductase and glycerol dehydratase, two key enzyme catalyzing reactions of 3-HPA formation and consumption, were sensitive to high concentration of 3-HPA. When the concentration of 3-HPA increased to a higher level in medium (ac 10 mmol/L), the activity of 1,3-propanediol oxidoreductase in cell decreased correspondingly, which led to decrease of the 3-HPA conversion rate, then the 3-HPA concentration increasing was accelerated furthermore. 3-HPA accumulation in culture medium was triggered by this positive feedback mechanism. In the cell exponential growth phase, the reaction catalyzed by 1,3-propanediol oxidoreductase was the rate limiting step in 1,3-propanediol production. The level of 3-HPA in culture medium could be controlled by the substrate (glycerol) concentration, and lower level of glycerol could avoid 3-HPA accumulating to a high, lethal concentration. In fed batch fermentation, under the condition of initial glycerol concentration 30 g/L, and keeping glycerol concentration lower than 7-8 g/L in cell exponential growth phase, 3-HPA accumulation could not be incurred. Based on this result, a glycerol feeding strategy was set up in fed batch fermentation. Under the optimized condition, 50.1 g/L of 1,3-propanediol was produced in 24 h, and 73.1 g/L of final 1,3-propanediol concentration was obtained in 54 h.
语种	英语 ; 英语
出版者	SPRINGER HEIDELBERG ; HEIDELBERG ; TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17562]
专题	清华大学
推荐引用方式 GB/T 7714	Hao, Jian,Lin, Rihui,Zheng, Zongming,et al. 3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae[J],2010, 2010.
APA	Hao, Jian,Lin, Rihui,Zheng, Zongming,Sun, Yan,&Liu, Dehua.(2010).3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae..
MLA	Hao, Jian,et al."3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae".(2010).

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