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	Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design
	Zheng, Zong-Ming ; Hu, Qiu-Iong ; Hao, Jian ; Xu, Feng ; Guo, Ni-Ni ; Sun, Yan ; Liu, De-Hua
	2010-05-07 ; 2010-05-07
关键词	RSM CCD 1,3-PD optimization Klebsiella pneumoniae RESPONSE-SURFACE METHODOLOGY FERMENTATION GLYCEROL BATCH Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
中文摘要	A central composite design was used to study the effect of glycerol, rate of stirring, air aeration and pH on the synthesis of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae AC 15. Among the four variables, glycerol and rate of stirring significantly affected 1,3-PD productivity, whereas air aeration and pH were not effective. A quadratic polynomial equation was obtained for 1,3-PD productivity by multiple regression analysis using response surface methodology. The validation experimental confirmed with the predicted model. The optimum combinations for 1,3-PD productivity was glycerol, rate of stirring, air aeration, and pH of 50 g/l, 318 rpm, 0.6 vvm, 6.48, respectively. The subsequent fed batch experiments produced 1,3-PD of 70 g/l at a fermentation of 30 h. (c) 2007 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17299]
专题	清华大学
推荐引用方式 GB/T 7714	Zheng, Zong-Ming,Hu, Qiu-Iong,Hao, Jian,et al. Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design[J],2010, 2010.
APA	Zheng, Zong-Ming.,Hu, Qiu-Iong.,Hao, Jian.,Xu, Feng.,Guo, Ni-Ni.,...&Liu, De-Hua.(2010).Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design..
MLA	Zheng, Zong-Ming,et al."Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design".(2010).

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