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	A mathematical model for regulating monomer composition of the microbially synthesized polyhydroxyalkanoate copolymers
	Xu, J ; Guo, BH ; Zhang, ZM ; Wu, Q ; Zhou, Q ; Chen, JC ; Chen, GQ ; Li, GD
	2010-05-07 ; 2010-05-07
关键词	polyhydroxyalkanoate copolymers monomer composition model genetic engineering PSEUDOMONAS-STUTZERI 1317 RECOMBINANT ESCHERICHIA-COLI FED-BATCH CULTURE RALSTONIA-EUTROPHA BIODEGRADABLE PLASTICS ALCALIGENES-EUTROPHUS UNSATURATED MONOMERS SYNTHASE GENES CARBON-SOURCES PHA SYNTHASE Biotechnology & Applied Microbiology
中文摘要	A mathematical model is proposed for predicting the copolymer composition of the microbially synthesized polyhydroxyalkanoate (PHA) copolymers. Based on the biochemical reactions involved in the precursor formation and polymerization pathways, the model correlates the copolymer composition with the cultivation conditions, the enzyme levels and selectivity, and the metabolic pathways. It suggests the following points: (1) in the case of a sole carbon source, the copolymer composition depends mainly on the topology of the metabolic pathways and the selectivity of both the enzymes involved in the precursor formation and the polymerization route; (2) the copolymer composition can be varied in a wide range via alteration of the flux ratio of different types of monomers channeled from two or more independent and simultaneous pathways; (3) the enzymes which should be over-expressed or inhibited to obtain the desired copolymer composition can be predicted. For example, inhibition of the beta-oxidation pathway will increase the content of the monomer units with longer chain length. To test the model, various experiments were envisaged by varying cultivation time, concentration and chain length of the sole carbon source, and molar ratio of the cosubstrates. The predictions from the model agree well with the experimental results. Therefore, the proposed model will be useful in predicting the PHA copolymer composition under different biochemical reaction conditions. In other words, it can provide a guide for the synthesis of desired PHA copolymers. (c) 2005 Wiley Periodicals, Inc.
语种	英语 ; 英语
出版者	JOHN WILEY & SONS INC ; HOBOKEN ; 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17271]
专题	清华大学
推荐引用方式 GB/T 7714	Xu, J,Guo, BH,Zhang, ZM,et al. A mathematical model for regulating monomer composition of the microbially synthesized polyhydroxyalkanoate copolymers[J],2010, 2010.
APA	Xu, J.,Guo, BH.,Zhang, ZM.,Wu, Q.,Zhou, Q.,...&Li, GD.(2010).A mathematical model for regulating monomer composition of the microbially synthesized polyhydroxyalkanoate copolymers..
MLA	Xu, J,et al."A mathematical model for regulating monomer composition of the microbially synthesized polyhydroxyalkanoate copolymers".(2010).

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