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	Morphological transformation of plant cells in vitro and its effect on plant growth
	Guo Zhigang ; Zeng Zhaolin ; Liu Ruizhi ; Deng Ying
	2010-05-07 ; 2010-05-07
关键词	Practical Experimental/ biotechnology cellular biophysics osmosis sugar/ morphological transformation plant growth cell growth suspension cultures plant cell culture engineering saffron cells Crocus sativus L unbalanced osmotic pressure sucrose concentration cytoplasm shrinkage vacuolation ionic strength inoculation ratio/ A8725 Cellular biophysics A8265F Film and membrane processes ion exchange dialysis osmosis, electro-osmosis E3628 Biotechnology industry E1525 Industrial processes
中文摘要	Enhancement of cell growth in suspension cultures is urgently needed in plant cell culture engineering. This study investigates the relationship between morphological transformation and cell growth in callus and suspension cultures of saffron cells belonging to the cell line C96 induced from Crocus sativus L. In the suspension culture, an unbalanced osmotic pressure between the intracell and extracell regions induced a large morphological transformation which affected normal division of the saffron cells. An increase in osmotic pressure caused by the addition of sucrose inhibits the vacuolation and shrinkage of cytoplasm in the cells. As the sucrose concentration increases, the total amount of accumulated biomass also increases. Besides the sucrose concentration, increased ionic strength and inoculation ratio also help restrain to a large extent the vacuolation and shrinkage of the cytoplasm in the suspended cells, which results in increased biomass. The conditions for optimal biomass are: Murashige and Skoog's (MS) medium with 40 g/L sucrose and 60% (vlv) inoculation ratio.
语种	英语 ; 英语
出版者	Editorial Board of J. of Tsinghua University ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17266]
专题	清华大学
推荐引用方式 GB/T 7714	Guo Zhigang,Zeng Zhaolin,Liu Ruizhi,et al. Morphological transformation of plant cells in vitro and its effect on plant growth[J],2010, 2010.
APA	Guo Zhigang,Zeng Zhaolin,Liu Ruizhi,&Deng Ying.(2010).Morphological transformation of plant cells in vitro and its effect on plant growth..
MLA	Guo Zhigang,et al."Morphological transformation of plant cells in vitro and its effect on plant growth".(2010).

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