Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis | |
Gou, Zhongxuan ; Xing, Xin-Hui ; Luo, Mingfang ; Jiang, Hao ; Han, Bing ; Wu, Hao ; Wang, Lei ; Zhang, Fei | |
2010-05-07 ; 2010-05-07 ; OCT | |
关键词 | particulate methane mono-oxygenase Rhodococcus erythropolis functional expression Methylosinus trichosporium OB3b METHYLOSINUS-TRICHOSPORIUM OB3B METHYLOCOCCUS-CAPSULATUS BATH COPPER IONS METHANOTROPHS BIOREMEDIATION IDENTIFICATION OXIDATION PROTEIN Microbiology |
中文摘要 | In order to construct an expression system for the particulate methane monooxygenase (AMMO) gene (pmo), the structural gene cluster pmoCAB amplified from Methylosinus trichosporium OB3b was inserted into a shuttle vector pBS305 under the control of a dsz promoter and transformed into Rhodococcus erythropolis LSSE8-1. A stable transformant was successfully obtained using ethane as the sole carbon source. Fluorescence in situ hybridization results showed that the dsz promoter allowed the pmo genes to be transcribed in the recombinant strain. The effects of Cu2+ and Zn2+ concentrations on cell growth and pMMO activity in ethane-containing medium were examined. It was discovered that 7.5 mu M Cu2+ and 1.8 mu M Zn2+ were suitable to achieve high cell concentration and pMMO activity, but the amount of methanol accumulated during methane oxidation by the recombinant strain was still low. |
语种 | 英语 ; 英语 |
出版者 | BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/17264] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Gou, Zhongxuan,Xing, Xin-Hui,Luo, Mingfang,et al. Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis[J],2010, 2010, OCT. |
APA | Gou, Zhongxuan.,Xing, Xin-Hui.,Luo, Mingfang.,Jiang, Hao.,Han, Bing.,...&Zhang, Fei.(2010).Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis.. |
MLA | Gou, Zhongxuan,et al."Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis".(2010). |
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