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	Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis
	Gou, Zhongxuan ; Xing, Xin-Hui ; Luo, Mingfang ; Jiang, Hao ; Han, Bing ; Wu, Hao ; Wang, Lei ; Zhang, Fei
	2010-05-07 ; 2010-05-07 ; OCT
关键词	particulate methane mono-oxygenase Rhodococcus erythropolis functional expression Methylosinus trichosporium OB3b METHYLOSINUS-TRICHOSPORIUM OB3B METHYLOCOCCUS-CAPSULATUS BATH COPPER IONS METHANOTROPHS BIOREMEDIATION IDENTIFICATION OXIDATION PROTEIN Microbiology
中文摘要	In order to construct an expression system for the particulate methane monooxygenase (AMMO) gene (pmo), the structural gene cluster pmoCAB amplified from Methylosinus trichosporium OB3b was inserted into a shuttle vector pBS305 under the control of a dsz promoter and transformed into Rhodococcus erythropolis LSSE8-1. A stable transformant was successfully obtained using ethane as the sole carbon source. Fluorescence in situ hybridization results showed that the dsz promoter allowed the pmo genes to be transcribed in the recombinant strain. The effects of Cu2+ and Zn2+ concentrations on cell growth and pMMO activity in ethane-containing medium were examined. It was discovered that 7.5 mu M Cu2+ and 1.8 mu M Zn2+ were suitable to achieve high cell concentration and pMMO activity, but the amount of methanol accumulated during methane oxidation by the recombinant strain was still low.
语种	英语 ; 英语
出版者	BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17264]
专题	清华大学
推荐引用方式 GB/T 7714	Gou, Zhongxuan,Xing, Xin-Hui,Luo, Mingfang,et al. Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis[J],2010, 2010, OCT.
APA	Gou, Zhongxuan.,Xing, Xin-Hui.,Luo, Mingfang.,Jiang, Hao.,Han, Bing.,...&Zhang, Fei.(2010).Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis..
MLA	Gou, Zhongxuan,et al."Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis".(2010).

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