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	Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01
	Hao, Jian ; Wang, Wei ; Tian, Jiesheng ; Li, Jilun ; Liu, Dehua
	2010-05-07 ; 2010-05-07
关键词	dhaT 1,3-propanediol 3-hydroxypropionaldehyde Klebsiella pneumoniae CITROBACTER-FREUNDII CONTINUOUS-CULTURE GLYCEROL FERMENTATION DISSIMILATION CONSTRUCTION CLONING Biotechnology & Applied Microbiology
中文摘要	Glycerol can be biologically converted to 1,3-propanediol, a key raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers. In 1,3-propanediol synthesis pathway, 3-hydroxypropionaldehyde (3-HPA) was an inhibitory intermediary metabolite. The accumulation of 3-HPA in broth would cause an irreversible cessation of the fermentation process. With the object of reducing 3-HPA level in the fermentation broth, dhaT gene which encodes 1,3-propanediol oxidoreductase (PDOR) was cloned and over expressed in 1,3-propanediol producing bacterium Klebsiella pneumoniae TUAC01. dhaT gene was linked downstream of the ptac promoter in an expressing vector pDK6 to form plasmid pDK-dhaT. The newly formed pDK-dhaT was transformed to K. pneumoniae TUAC01. Under the inducement of IPTG, PDOR was over-expressed when the constructed strain was cultured on an LB medium or a fermentation medium. A 5 L scale-up fermentation experiment was done to test the 3-HPA accumulation in broth, with the initial substrate glycerol 30 g/L; the peak levels of 3-HPA in broth were 7.55 and 1.49 mmol/L for control host strain and the constructed strain, respectively. In 50 g/L initial glycerol experiment, the peak level of 3-HPA in broth was 12.57 and 2.02 mmol/l for the control host strain and the constructed strain, respectively. Thus the fermentation cessation caused by the toxicity of 3-HPA was alleviated in the constructed strain.
语种	英语 ; 英语
出版者	SPRINGER HEIDELBERG ; HEIDELBERG ; TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17208]
专题	清华大学
推荐引用方式 GB/T 7714	Hao, Jian,Wang, Wei,Tian, Jiesheng,et al. Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01[J],2010, 2010.
APA	Hao, Jian,Wang, Wei,Tian, Jiesheng,Li, Jilun,&Liu, Dehua.(2010).Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01..
MLA	Hao, Jian,et al."Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01".(2010).

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