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	Engineering of vascular grafts with genetically modified bone marrow mesenchymal stem cells on poly (propylene carbonate) graft
	Zhang, Jun ; Qi, Hongxu ; Wang, Hongjun ; Hu, Ping ; Ou, Lailiang ; Guo, Shuhua ; Li, Jing ; Che, Yongzhe ; Yu, Yaoting ; Kong, Deling
	2010-05-07 ; 2010-05-07
关键词	bone marrow stem cells tissue engineering vascular graft endothelial nitric oxide synthase gene therapy ENDOTHELIAL PROGENITOR CELLS OXIDE SYNTHASE GENE NEOINTIMAL HYPERPLASIA BLOOD-VESSEL POLYTETRAFLUOROETHYLENE ARTERIES PATENCY MATRIX VIVO Engineering, Biomedical Transplantation
中文摘要	Bone marrow mesenchymal stem cells (MSCs) have demonstrated their pluripotency to differentiate into different cell lineages and may be an alternative cell source for vascular tissue engineering. The objective of this study is to create small diameter vessels by seeding and culture of genetically modified MSCs onto a synthetic polymer scaffold produced by an electrospinning technique. A tubular scaffold (2 mm in diameter) with a microstructure of nonwoven fibers was produced by electrospinning of poly (propylene carbonate) (PPC). Rat MSCs obtained from bone marrow were expanded in culture and modified with vasculoprotective gene endothelial nitric oxide synthase (eNOS) or marker gene green fluorescent protein (GFP). These MSCs were seeded onto the electrospun fibrous grafts (internal diameter = 2 mm), and cultured in 5% CO2 at 37 degrees C. The growth of MSCs in the scaffold was analyzed with scanning electron microscopy (SEM) and hematoxylin and eosin (H&E) staining. The gene transfer and transgenic gene expression were examined with fluorescence-activated cell sorting (FACS), immunochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR), and western blot. The production of nitric oxide (NO) by the engineered vessels was measured with an NO detection kit. Our data showed that the seeded cells integrated with the microfibers of the scaffold to form a three-dimensional cellular network, indicating a favorable interaction between this synthetic PPC scaffold with MSCs. High transduction efficiency was obtained with the use of concentrated retrovirus in the gene transfection of MSCs. The eNOS gene transcripts and protein were detected in the grafts seeded with eNOS-modified MSCs by RT-PCR and immunochemical staining. The amount of NO produced by grafts seeded with eNOS-modified MSCs was comparable to that produced by native blood vessels, and it was significantly higher than that in the grafts seeded with nonmodified MSCs. In summary, the vascular graft produced by culture of eNOS gene-modified MSCs onto the electrospun tubular scaffolds shows promising results in terms of function. The use of MSCs and therapeutic genes in tissue engineering of blood vessels could be helpful in improving vessel regeneration and patency.
语种	英语 ; 英语
出版者	BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/17190]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Jun,Qi, Hongxu,Wang, Hongjun,et al. Engineering of vascular grafts with genetically modified bone marrow mesenchymal stem cells on poly (propylene carbonate) graft[J],2010, 2010.
APA	Zhang, Jun.,Qi, Hongxu.,Wang, Hongjun.,Hu, Ping.,Ou, Lailiang.,...&Kong, Deling.(2010).Engineering of vascular grafts with genetically modified bone marrow mesenchymal stem cells on poly (propylene carbonate) graft..
MLA	Zhang, Jun,et al."Engineering of vascular grafts with genetically modified bone marrow mesenchymal stem cells on poly (propylene carbonate) graft".(2010).

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