A comparison between photon counting histogram and fluorescence intensity distribution analysis | |
Meng, Fanbo ; Ma, Hui | |
2010-05-06 ; 2010-05-06 | |
关键词 | CORRELATION SPECTROSCOPY FLUCTUATION SPECTROSCOPY EXCITATION CELL Chemistry, Physical |
中文摘要 | Photon counting histogram (PCH) and fluorescence intensity distribution analysis (FIDA) are two methods that were developed independently but reported almost simultaneously. Both of them have been successfully applied to fluorescence fluctuation spectroscopy (FFS). Though publications have indicated that they are theoretically equivalent, they are still commonly considered as different methods, especially in their ways to treat the point spread functions (PSFs). In this paper, the two methods are examined in detail for a direct comparison. After a direct proof of the theoretical equivalence, the authors further point out that PCH and FIDA are completely equivalent in the way of modeling PSFs; that is, any modeling approach developed from one of them can always be applied to the other. It is also demonstrated that simplified FIDA and PCH formulas in the form of power series can be applied for fast and precise numerical calculations. The two methods are also compared for their merits in the calculation efficiency. |
语种 | 英语 ; 英语 |
出版者 | AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/14423] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Meng, Fanbo,Ma, Hui. A comparison between photon counting histogram and fluorescence intensity distribution analysis[J],2010, 2010. |
APA | Meng, Fanbo,&Ma, Hui.(2010).A comparison between photon counting histogram and fluorescence intensity distribution analysis.. |
MLA | Meng, Fanbo,et al."A comparison between photon counting histogram and fluorescence intensity distribution analysis".(2010). |
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