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	Copper binding properties of a tau peptide associated with Alzheimer's disease studied by CD, NMR, and MALDI-TOF MS
	Ma, QF ; Li, YM ; Du, JT ; Liu, HD ; Kanazawa, K ; Nemoto, T ; Nakanishi, H ; Zhao, YF
	2010-05-06 ; 2010-05-06
关键词	CD spectra copper binding MALDI-TOF MS NMR spectroscopy R2 peptide tau protein PAIRED HELICAL FILAMENTS PROTEIN-TAU IN-VITRO BETA-STRUCTURE ION COMPLEXES AMINO ACIDS ALPHA-HELIX BRAIN COORDINATION AGGREGATION Biochemistry & Molecular Biology Pharmacology & Pharmacy
中文摘要	We have previously reported the copper binding properties of R3 peptide (residues 318-335: VTSKCGSLGNIHHKPGGG, according to the longest tau protein) derived from the third repeat microtubule-binding domain of water-soluble tau protein. In this work, we have investigated copper binding properties of R2 peptide (residues 287-304: VQSKCGSKDNIKHVPGGG) derived from the second repeat region of tau protein. Similar to R3 peptide, R2 peptide also plays an important role in the formation of neurofibrillary tangles (NFTs) which is one of the two main biological characteristics of Alzheimer's disease (AD). Based on the copper binding properties of R2 peptide, the possible influences of the binding on the formation of NFTs were investigated. Results from circular dichroism (CD) spectra, nuclear magnetic resonance (NMR) spectroscopy, and matrix assisted laser desorptior/ionization time of flight mass spectrometry (MALDI-TOF MS) suggest that the binding is pH-dependent and stoichiometry-determined. In addition, these results also reveal that R2 peptide adopts a monomeric alpha-helical structure in aqueous solutions at physiological pH after the addition of 1 mol equiv. of Cu2+. Since a-helix structure is responsible for the formation of paired helical filaments (PHFs) which aggregate into NFTs, it is hypothesized that Cu2+ induces R2 peptide to self-assemble into a PHFs-like structure. Hence, it is postulated that Cu2+ plays an important role in the aggregation of R2 peptide and tau protein and that copper binding to R2 peptide may be another possible involvement in AD. (c) 2005 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13649]
专题	清华大学
推荐引用方式 GB/T 7714	Ma, QF,Li, YM,Du, JT,et al. Copper binding properties of a tau peptide associated with Alzheimer's disease studied by CD, NMR, and MALDI-TOF MS[J],2010, 2010.
APA	Ma, QF.,Li, YM.,Du, JT.,Liu, HD.,Kanazawa, K.,...&Zhao, YF.(2010).Copper binding properties of a tau peptide associated with Alzheimer's disease studied by CD, NMR, and MALDI-TOF MS..
MLA	Ma, QF,et al."Copper binding properties of a tau peptide associated with Alzheimer's disease studied by CD, NMR, and MALDI-TOF MS".(2010).

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