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	Direct electrochemistry and electrocatalysis based on film of horseradish peroxidase intercalated into layered titanate nano-sheets
	Zhang, Ling ; Zhang, Qian ; Lu, Xianbo ; Li, Jinghong
	2010-05-06 ; 2010-05-06
关键词	direct electrochemistry electrocatalysis biosensor titanate nano-sheets horseradish peroxidase DIRECT ELECTRON-TRANSFER GLASSY-CARBON ELECTRODE ZIRCONIUM-PHOSPHATE CLAY NANOPARTICLES CATALYTIC-ACTIVITY IMMOBILIZATION HEMOGLOBIN BIOSENSORS NANOTUBES DEPOSITION Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology
中文摘要	Intercalation of horseradish peroxidase (HRP) into layered titanate by assembling it with titanate nano-sheets (TNS) was firstly used for fabrication of enzyme electrode (HRP-TNS electrode). XRD result revealed that HRP-TNS film featured layered structure with HRP monolayer intercalated between the titanate layers. UV-vis spectra result indicated the intercalated HRP in TNS film well retained its native structure. The HRP-TNS film was uniform with porous structures which were confirmed by SEM. The immobilized HRP in the TNS film exhibited fast direct electron transfer and showed a good electrocatalytic performance to H2O2 with high sensitivity, wide linear range and low detection. The excellent electrochemical performance of the HRP-TNS electrode was attributed to biocompatibility of the titanate sheets, porous architectures of the HRP-TNS film which retained activity of HRP to large extent, avoided aggregation of HRP, provided better mass transport and allowed more HRP loading per unit area. Thus, the simple method described here provides a novel and effective platform for immobilization of enzyme in realizing direct electrochemistry and has a promising application in fabrication of the third-generation electrochemical biosensors. (C) 2007 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER ADVANCED TECHNOLOGY ; OXFORD ; OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13613]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Ling,Zhang, Qian,Lu, Xianbo,et al. Direct electrochemistry and electrocatalysis based on film of horseradish peroxidase intercalated into layered titanate nano-sheets[J],2010, 2010.
APA	Zhang, Ling,Zhang, Qian,Lu, Xianbo,&Li, Jinghong.(2010).Direct electrochemistry and electrocatalysis based on film of horseradish peroxidase intercalated into layered titanate nano-sheets..
MLA	Zhang, Ling,et al."Direct electrochemistry and electrocatalysis based on film of horseradish peroxidase intercalated into layered titanate nano-sheets".(2010).

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