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	A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting
	Bo Yao ; Guo-an Luo ; Xue Feng ; Wei Wang ; Ling-xin Chen ; Yi-ming Wang
	2010-05-06 ; 2010-05-06
关键词	Practical Experimental/ biological effects of ultraviolet radiation biological techniques biomembranes cellular biophysics fluorescence microfluidics organic compounds/ microfluidic device gravity driving electric force driving flow cytometry fluorescence activated cell sorting microfluidic chip system microchip switch-off activation program microchannel electric field Newton law of motion hydroxylpropylmethyl cellulose HPMC necrotic effects apoptotic effects ultraviolet light HeLa cells UV radiation membrane damage apoptosis necrosis/ A8780 Biophysical instrumentation and techniques A8750G Biological effects of ionizing radiations (UV, X-ray, gamma-ray particle radiation effects) A8725F Physics of subcellular structures A8720 Membrane biophysics A0710C Micromechanical devices and systems B2575D Design and modelling of micromechanical devices
中文摘要	A novel method based on gravity and electric force driving of cells was developed for flow cytometry and fluorescence activated cell sorting in a microfluidic chip system. In the experiments cells flowed spontaneously under their own gravity in a upright microchip, passed through the detection region and then entered into the sorting electric field one by one at an average velocity of 0.55 mm s/sup -1/ and were fluorescence activated cell sorted (FACS) by a switch-off activation program. In order to study the dynamical and kinematic characteristics of single cells in gravity and electric field of microchannels a physical and numerical module based on Newton's law of motion was established and optimized. Hydroxylpropylmethyl cellulose (HPMC) was used to minimize cell assembling, sedimentation and adsorption to microchannels. This system was applied to estimate the necrotic and apoptotic effects of ultraviolet (UV) light on HeLa cells by exposing them to UV radiation for 10, 20 or 40 min and the results showed that UV radiation induced membrane damage contributed to the apoptosis and necrosis of HeLa cells.
语种	英语 ; 英语
出版者	R. Soc. Chem ; UK
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13534]
专题	清华大学
推荐引用方式 GB/T 7714	Bo Yao,Guo-an Luo,Xue Feng,et al. A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting[J],2010, 2010.
APA	Bo Yao,Guo-an Luo,Xue Feng,Wei Wang,Ling-xin Chen,&Yi-ming Wang.(2010).A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting..
MLA	Bo Yao,et al."A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting".(2010).

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