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	Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum
	Wang, Xu ; Chen, Hui ; Lin, Jin-Ming ; Ying, Xitang
	2010-05-06 ; 2010-05-06
关键词	free thyroxine (FT4) horseradish peroxidase (HRP) chemiluminescence enzyme immunoassay (CLEIA) POLYACRYLAMIDE-GEL FILTRATION THYROID-FUNCTION ABNORMAL CONCENTRATIONS ULTRAFILTRATION METHOD NONTHYROIDAL ILLNESS EQUILIBRIUM DIALYSIS REFERENCE INTERVALS MOLECULAR-WEIGHT TRIIODOTHYRONINE THYROTROPIN Biochemistry & Molecular Biology Biology
中文摘要	A microplate chemiluminescence enzyme immunoassay (CLEIA) with high sensitivity, selectivity and reproducibility was developed for the determination of free thyroxine (FT4) in human serum. A competitive assay has been utilized with horseradish peroxidase (HRP) labeled thyroxine analog in the chemiluminescence (CL) detection. The CL signal produced by the emission of photons from luminol was directly proportional to the amount of analyte. The linear range was 0.45-7.5 ng dL(-1) and the detection limit was 0.09 ng dL(-1). Experimental conditions, such as temperature, pH, incubation time, titration level and other relevant variables upon the CL signal have been examined and optimized. A coefficient of variance of less than 16% was obtained for intra- and inter-assay precision. The present method has been successfully applied to the analysis of FT4 in human serum. The positive and negative coincidence ratios are satisfactory. Good correlations were obtained between the results by the proposed method and radioimmunoassay (RIA), as well as a Bayer ACS-180SE detection system.
语种	英语 ; 英语
出版者	IVYSPRING INT PUBL ; LAKE HAVEN ; PO BOX 4546, LAKE HAVEN, NSW 2263, AUSTRALIA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13331]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Xu,Chen, Hui,Lin, Jin-Ming,et al. Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum[J],2010, 2010.
APA	Wang, Xu,Chen, Hui,Lin, Jin-Ming,&Ying, Xitang.(2010).Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum..
MLA	Wang, Xu,et al."Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum".(2010).

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