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	Effect of the phosphate group with different negative charges on the conformation of phosphorylated Ser/Thr-Pro motif
	Guo, YT ; Li, YM ; Zhu, ZT ; Zhao, YF
	2010-05-06 ; 2010-05-06
关键词	cis/trans isomerization conformational study LC-MS NMR phosphorylation proline PROLINE-DIRECTED PHOSPHORYLATION CIS-TRANS ISOMERIZATION ALZHEIMERS-DISEASE PEPTIDES ANALOGS BINDING TAU Biochemistry & Molecular Biology
中文摘要	Ser/Thr-Pro motif is a widespread phosphorylated site in proteins, and its reversible phosphorylation is an important regulatory progress in many cell cycles and signal transduction. Recent research reveals that phosphorylation affects the local conformation of the peptide and its binding with the substrate through peptidyl-prolyl cis/trans isomerization. In order to further explore the effect of the phosphate group with different charges, four model peptides containing non- and phosphorylated Ser/Thr-Pro motif were synthesized using the classical solid-phase method. 1H-NMR, TOCSY, and ROESY were employed to characterize the conformation of the model peptides in solution with different pH value and analyze the peptidyl-prolyl isomerization at a molecular level. The results demonstrate that phosphorylation increases the cis conformation in the peptide and the maximum cis/traps ratio is obtained when the phosphate group has two negative charges. Furthermore, the experiments prove that the phosphorylation introduces a hydrogen bond between the phosphate and the NH of Ser/Thr residue, and the charges of the phosphate affect certain conformations of the phosphorylated Ser/Thr-Pro motif.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING STREET, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13308]
专题	清华大学
推荐引用方式 GB/T 7714	Guo, YT,Li, YM,Zhu, ZT,et al. Effect of the phosphate group with different negative charges on the conformation of phosphorylated Ser/Thr-Pro motif[J],2010, 2010.
APA	Guo, YT,Li, YM,Zhu, ZT,&Zhao, YF.(2010).Effect of the phosphate group with different negative charges on the conformation of phosphorylated Ser/Thr-Pro motif..
MLA	Guo, YT,et al."Effect of the phosphate group with different negative charges on the conformation of phosphorylated Ser/Thr-Pro motif".(2010).

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