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	Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanoparticle labeled DNA as probe onto titanium dioxide electrode
	Lu, Wu ; Jin, Yan ; Wang, Geng ; Chen, Da ; Li, Jinghong
	2010-05-06 ; 2010-05-06
关键词	DNA sensor photoelectrochemical cell nanoparticles SIGNAL AMPLIFICATION NANOPOROUS TIO2 CHARGE-TRANSFER HOLE TRANSPORT FILMS NANOSTRUCTURES BIOSENSORS SENSORS Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology
中文摘要	A photoelectrochemical method was proposed to detect DNA hybridization using Au narroparticle modified DNA as one probe on TiO2 substrate, in which the TiO2 substrate was used not only as DNA anchors but also as the signal transducers. Hybridization between the probe and the target DNA oligonucleotides was confirmed by the decreased photocurrent of the TiO2 electrode. Compared with non-label probe, Au nanoparticles enhanced the photocurrent shifts after the hybridization. The photocurrent decreased with increasing the concentration of target DNA, indicating that this method could be used for quantitative measurements, and the discrimination of the complementary from mismatched DNA. Furthermore, the hybridization binding constant was obtained and photocurrent generation mechanism was discussed. The major advantages of this photochemical method are speed, simplicity and excellent specificity. This method provides a platform for studying a wide variety of biological processes using photoelectrochemical method. (C) 2008 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER ADVANCED TECHNOLOGY ; OXFORD ; OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/12696]
专题	清华大学
推荐引用方式 GB/T 7714	Lu, Wu,Jin, Yan,Wang, Geng,et al. Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanoparticle labeled DNA as probe onto titanium dioxide electrode[J],2010, 2010.
APA	Lu, Wu,Jin, Yan,Wang, Geng,Chen, Da,&Li, Jinghong.(2010).Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanoparticle labeled DNA as probe onto titanium dioxide electrode..
MLA	Lu, Wu,et al."Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanoparticle labeled DNA as probe onto titanium dioxide electrode".(2010).

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