| Determination of free triiodothyronine by chemiluminescent enzyme immunoassay | |
| Lin Zhen ; Wang Xu ; Ren Si-Qi ; Chen Guo-Nan ; Li Zhen-Jia ; Lin Jin-Ming | |
| 2010-05-06 ; 2010-05-06 | |
| 关键词 | chemiluminescent enzyme immunoassay free triiodothyronine analog THYROXINE-BINDING GLOBULIN SERUM RADIOIMMUNOASSAY VALIDATION ASSAYS Chemistry, Analytical |
| 中文摘要 | The methodology based on chemiluminescent enzyme immunoassay was established for the determination of free triiodothyronine (FT3). The HRP-luminol-H2O2 chemiluminescent system with high sensitivity was chosen as the detection system. The linear range was 0.90-80 ng/L. Under the selected condition, the RSDs of intra- and inter-assays were less than 15%. When the method was applied to detect free triiodothyronine in human serum, the diagnose accordance rate of the method was 83.3%. Compared with the commercial kit, the correlation coefficient was 0.9005. All the conditions chosen are to maintain the equilibrium between the free and the bounded hormones in order to improve the validity of the experimental result. |
| 语种 | 中文 ; 中文 |
| 出版者 | SCIENCE CHINA PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/12549]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Lin Zhen,Wang Xu,Ren Si-Qi,et al. Determination of free triiodothyronine by chemiluminescent enzyme immunoassay[J],2010, 2010. |
| APA | Lin Zhen,Wang Xu,Ren Si-Qi,Chen Guo-Nan,Li Zhen-Jia,&Lin Jin-Ming.(2010).Determination of free triiodothyronine by chemiluminescent enzyme immunoassay.. |
| MLA | Lin Zhen,et al."Determination of free triiodothyronine by chemiluminescent enzyme immunoassay".(2010). |
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