| Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry | |
| Liu, HD ; Li, YM ; Du, JT ; Hu, J ; Zhao, YF | |
| 2010-05-06 ; 2010-05-06 | |
| 关键词 | UDP-GlcNAc rearrangement reaction pentacoordinated phosphoric intermediate migration electrospray ionization tandem mass spectrometry DYNAMIC O-GLYCOSYLATION CYTOSOLIC PROTEINS STRUCTURAL-CHARACTERIZATION RESIDUE LOSS C-MYC GLCNAC BETA PHOSPHORYLATION SPECTRA NUCLEAR Biophysics Chemistry, Organic Spectroscopy |
| 中文摘要 | Uridine 5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) is the final product of hexosamine biosynthetic pathway (HSP) and the donor substrate for the modification of nucleocytoplasmic proteins at serine and threonine residues with N-acetylglucosamine (GlcNAc) catalyzed by O-GlcNAc transferase (OGT). Many analogs of UDP-GlcNAc were designed to interfere with the process of protein O-glycosylation by blocking OGT. A novel rearrangement reaction was observed in which phosphate-N-acetylglucosamine moiety migrated to 3' terminus of ribose in ESI-MSn of UDP-GlcNAc. Results from tandem mass spectrometry, control experiments and calculation showed that the phosphate-N-acetylglucosamine migration might undergo a pentacoordinate phosphoric intermediate. Furthermore, the acetylation of glucosamine in UDP-GlcNAc was essential in the migration process. Copyright (c) 2005 John Wiley & Sons, Ltd. |
| 语种 | 英语 ; 英语 |
| 出版者 | JOHN WILEY & SONS LTD ; CHICHESTER ; THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/12499]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Liu, HD,Li, YM,Du, JT,et al. Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry[J],2010, 2010. |
| APA | Liu, HD,Li, YM,Du, JT,Hu, J,&Zhao, YF.(2010).Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry.. |
| MLA | Liu, HD,et al."Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry".(2010). |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论