Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry | |
Liu, HD ; Li, YM ; Du, JT ; Hu, J ; Zhao, YF | |
2010-05-06 ; 2010-05-06 | |
关键词 | UDP-GlcNAc rearrangement reaction pentacoordinated phosphoric intermediate migration electrospray ionization tandem mass spectrometry DYNAMIC O-GLYCOSYLATION CYTOSOLIC PROTEINS STRUCTURAL-CHARACTERIZATION RESIDUE LOSS C-MYC GLCNAC BETA PHOSPHORYLATION SPECTRA NUCLEAR Biophysics Chemistry, Organic Spectroscopy |
中文摘要 | Uridine 5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) is the final product of hexosamine biosynthetic pathway (HSP) and the donor substrate for the modification of nucleocytoplasmic proteins at serine and threonine residues with N-acetylglucosamine (GlcNAc) catalyzed by O-GlcNAc transferase (OGT). Many analogs of UDP-GlcNAc were designed to interfere with the process of protein O-glycosylation by blocking OGT. A novel rearrangement reaction was observed in which phosphate-N-acetylglucosamine moiety migrated to 3' terminus of ribose in ESI-MSn of UDP-GlcNAc. Results from tandem mass spectrometry, control experiments and calculation showed that the phosphate-N-acetylglucosamine migration might undergo a pentacoordinate phosphoric intermediate. Furthermore, the acetylation of glucosamine in UDP-GlcNAc was essential in the migration process. Copyright (c) 2005 John Wiley & Sons, Ltd. |
语种 | 英语 ; 英语 |
出版者 | JOHN WILEY & SONS LTD ; CHICHESTER ; THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/12499] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Liu, HD,Li, YM,Du, JT,et al. Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry[J],2010, 2010. |
APA | Liu, HD,Li, YM,Du, JT,Hu, J,&Zhao, YF.(2010).Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry.. |
MLA | Liu, HD,et al."Novel acetylation-aided migrating rearrangement of uridine-diphosphate-N-acetylglucosamine in electrospray ionization multistage tandem mass spectrometry".(2010). |
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