Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification | |
Li, Hai-Fang ; Liu, Jiangjiang ; Cai, Zongwei ; Lin, Jin-Ming | |
2010-05-06 ; 2010-05-06 | |
关键词 | electrospray ionization microchip electrophoresis pepticles quadrupole time-of-flight mass spectrometer CAPILLARY ELECTROPHORESIS MICROFABRICATED DEVICES PROTEIN IDENTIFICATION MICROFLUIDIC DEVICE DEAD VOLUME CHIP INTEGRATION INTERFACE SYSTEMS TIP Biochemical Research Methods Chemistry, Analytical |
中文摘要 | The present study reports a simple method of coupling a glass microchip to an electrospray ionization (ESI) quadrupole time-of-flight mass spectrometer (QTOF-MS) for separation and identification of peptides. A sheath-flow electrospray interface was constructed based on attaching a short fused-silica capillary to the microchip. The dead volume at the interface was effectively reduced by wet etching an approximate flat-bottom capillary insertion channel coaxial to the end of separation microchannel and using a wire-controlled epoxy-blocking attachment method. The makeup liquid and neb gas were coaxially pumped through two stainless-steel tees to maintain a stable and efficient electrospray. The coupled microchip/ESI-QTOF-MS system was successfully used to carry out electrophoresis separation of peptides and ESI-QTOF-MS identification. |
语种 | 英语 ; 英语 |
出版者 | WILEY-V C H VERLAG GMBH ; WEINHEIM ; PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/12391] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Li, Hai-Fang,Liu, Jiangjiang,Cai, Zongwei,et al. Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification[J],2010, 2010. |
APA | Li, Hai-Fang,Liu, Jiangjiang,Cai, Zongwei,&Lin, Jin-Ming.(2010).Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification.. |
MLA | Li, Hai-Fang,et al."Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification".(2010). |
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