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	Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification
	Li, Hai-Fang ; Liu, Jiangjiang ; Cai, Zongwei ; Lin, Jin-Ming
	2010-05-06 ; 2010-05-06
关键词	electrospray ionization microchip electrophoresis pepticles quadrupole time-of-flight mass spectrometer CAPILLARY ELECTROPHORESIS MICROFABRICATED DEVICES PROTEIN IDENTIFICATION MICROFLUIDIC DEVICE DEAD VOLUME CHIP INTEGRATION INTERFACE SYSTEMS TIP Biochemical Research Methods Chemistry, Analytical
中文摘要	The present study reports a simple method of coupling a glass microchip to an electrospray ionization (ESI) quadrupole time-of-flight mass spectrometer (QTOF-MS) for separation and identification of peptides. A sheath-flow electrospray interface was constructed based on attaching a short fused-silica capillary to the microchip. The dead volume at the interface was effectively reduced by wet etching an approximate flat-bottom capillary insertion channel coaxial to the end of separation microchannel and using a wire-controlled epoxy-blocking attachment method. The makeup liquid and neb gas were coaxially pumped through two stainless-steel tees to maintain a stable and efficient electrospray. The coupled microchip/ESI-QTOF-MS system was successfully used to carry out electrophoresis separation of peptides and ESI-QTOF-MS identification.
语种	英语 ; 英语
出版者	WILEY-V C H VERLAG GMBH ; WEINHEIM ; PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/12391]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Hai-Fang,Liu, Jiangjiang,Cai, Zongwei,et al. Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification[J],2010, 2010.
APA	Li, Hai-Fang,Liu, Jiangjiang,Cai, Zongwei,&Lin, Jin-Ming.(2010).Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification..
MLA	Li, Hai-Fang,et al."Coupling a microchip with electrospray ionization quadrupole time-of-flight mass spectrometer for peptide separation and identification".(2010).

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