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	Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum
	Jin Hui ; Wang Xu ; Xin TianBing ; Gao Peng ; Lin Jin-Ming ; Liang ShuXuan
	2010-05-06 ; 2010-05-06 ; OCT
关键词	chemiluminescence enzyme immunoassay tumor marker carbohydrate antigen 72-4 human serum MONOCLONAL-ANTIBODY B72.3 CA 72-4 CARCINOEMBRYONIC ANTIGEN GASTRIC-CARCINOMA CANCER PATIENTS OVARIAN-CANCER TUMOR-MARKERS TAG-72 RADIOIMMUNOASSAY MANAGEMENT Multidisciplinary Sciences
中文摘要	A highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) was developed for the quantitative evaluation of carbohydrate antigen 72-4 (CA72-4) in human serum, using luminol-H2O2 catalyzed by horseradish peroxidase (HRP) as the chemiluminescence system. The simple and quick determination was accomplished through a sandwich reaction mode. Several physicochemical parameters of the immunoreaction, including incubation conditions, antibody coating conditions, dilution ratio of anti-CA72-4-HRP conjugate, and chemiluminescence reaction time, were studied and optimized. The proposed method exhibited a linear range of 0-200 U/mL with correlation coefficient and detection limit of 0.9995 and 0.18 U/mL, respectively. The inter-assay and intra-assay coefficients of variation (CV) were both less than 10%. The average recovery of two clinical sera with low and high concentration CA72-4 was 99.3% and 98.7%, respectively. Normal tumor markers, including AFP, CEA, CA24-2, CA19-9 and CA15-3, did not cross-react with each other. The method's stability was evaluated by assessing its analytical performance after storing the immunoreagents at 4 degrees C and 37 degrees C for 7 days. Little difference was found, indicating satisfactory stability of the method. The present method has been successfully applied to the detection of CA72-4 human serum, and showed a good correlation with the commercially available ELISA kit (r(2) =0.9383). This method showed great potential in the fabrication of diagnostic kit for CA72-4, and could be well used in diagnosis of cancer in clinical practice.
语种	英语 ; 英语
出版者	SCIENCE CHINA PRESS ; BEIJING ; 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/12238]
专题	清华大学
推荐引用方式 GB/T 7714	Jin Hui,Wang Xu,Xin TianBing,et al. Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum[J],2010, 2010, OCT.
APA	Jin Hui,Wang Xu,Xin TianBing,Gao Peng,Lin Jin-Ming,&Liang ShuXuan.(2010).Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum..
MLA	Jin Hui,et al."Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum".(2010).

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