Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii

CORC > 清华大学

	Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii
	Wu JX(吴锦霞) ; Hu ZL(胡章立) ; Wang CG(王潮岗) ; Li SF(黎双飞) ; Lei AP(雷安平)
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008
	2012-04-27 ; 2012-04-27
关键词	expression efficiency green fluorescent protein(GFP) HSP70A-RBCS2 RBCS2 transformation efficiency
中文摘要	To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii,a high efficient expression vector was constructed.Green fluorescent protein(GFP) was expressed in C.reinhardtii under the control of promoters:RBCS2 and HSP70A-RBCS2.Efficiency of transformation and expression were compared between two transgenic algae:RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ.Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2,and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ.In addition,a threefold increase of GFP in Tran-Ⅱwas induced by heat shock at 40°C.All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C.reinhardtii.
语种	中文
其他责任者	Institute of Ecology and Environment ; College of Life Sciences ; Shenzhen University ; Shenzhen ; 518060 ; China
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/244041/1424]
专题	清华大学
推荐引用方式 GB/T 7714	Wu JX,Hu ZL,Wang CG,et al. Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008,2012, 2012.
APA	吴锦霞,胡章立,王潮岗,黎双飞,&雷安平.(2012).Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008.
MLA	吴锦霞,et al."Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008 (2012).