Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii | |
Wu JX(吴锦霞) ; Hu ZL(胡章立) ; Wang CG(王潮岗) ; Li SF(黎双飞) ; Lei AP(雷安平) | |
刊名 | http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008
![]() |
2012-04-27 ; 2012-04-27 | |
关键词 | expression efficiency green fluorescent protein(GFP) HSP70A-RBCS2 RBCS2 transformation efficiency |
中文摘要 | To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii,a high efficient expression vector was constructed.Green fluorescent protein(GFP) was expressed in C.reinhardtii under the control of promoters:RBCS2 and HSP70A-RBCS2.Efficiency of transformation and expression were compared between two transgenic algae:RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ.Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2,and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ.In addition,a threefold increase of GFP in Tran-Ⅱwas induced by heat shock at 40°C.All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C.reinhardtii. |
语种 | 中文 |
其他责任者 | Institute of Ecology and Environment ; College of Life Sciences ; Shenzhen University ; Shenzhen ; 518060 ; China |
内容类型 | 期刊论文 |
源URL | [http://ir.calis.edu.cn/hdl/244041/1424] ![]() |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Wu JX,Hu ZL,Wang CG,et al. Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008,2012, 2012. |
APA | 吴锦霞,胡章立,王潮岗,黎双飞,&雷安平.(2012).Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008. |
MLA | 吴锦霞,et al."Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=HYFW200803004&dbname=CJFQ2008 (2012). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论