A miniature laser speckle fluorescence sectioning microscope for cell imaging

CORC > 清华大学

	A miniature laser speckle fluorescence sectioning microscope for cell imaging
	Shao YH(邵永红) ; Li H(李恒) ; Wen Q(文侨) ; Wang Y(王岩) ; Qu JL(屈军乐) ; Niu HB(牛憨笨)
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010008&dbname=CJFQ2010
	2012-04-26 ; 2012-04-26
中文摘要	We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (DPSS) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary artery endothelial (BPAE) cells are obtained. The image quality is similar to that of the images acquired by standard laser scanning confocal microscope (LSCM). Compared with LSCM, the laser speckle fluorescence microscope (LSFM) presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.
语种	中文
其他责任者	Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province ; Institute of Optoelectronics ; Shenzhen University
内容类型	期刊论文
源URL	[http://ircloud.calis.edu.cn/hdl/244041/55]
专题	清华大学
推荐引用方式 GB/T 7714	Shao YH,Li H,Wen Q,et al. A miniature laser speckle fluorescence sectioning microscope for cell imaging[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010008&dbname=CJFQ2010,2012, 2012.
APA	邵永红,李恒,文侨,王岩,屈军乐,&牛憨笨.(2012).A miniature laser speckle fluorescence sectioning microscope for cell imaging.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010008&dbname=CJFQ2010.
MLA	邵永红,et al."A miniature laser speckle fluorescence sectioning microscope for cell imaging".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010008&dbname=CJFQ2010 (2012).