Improving the precision of fluorescence lifetime measurement using a streak camera

CORC > 清华大学

	Improving the precision of fluorescence lifetime measurement using a streak camera
	Li H(李恒) ; Shao YH(邵永红) ; Wang Y(王岩) ; Qu JL(屈军乐) ; Niu HB(牛憨笨)
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010005&dbname=CJFQ2010
	2012-04-26 ; 2012-04-26
中文摘要	Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a two-photon excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. A continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a Fabry-Perot etalon is used to calibrate the nonlinearity of the sweeping speed. Fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision o...
语种	中文
其他责任者	Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province ; Institute of Optoelectronics ; Shenzhen University
内容类型	期刊论文
源URL	[http://ircloud.calis.edu.cn/hdl/244041/54]
专题	清华大学
推荐引用方式 GB/T 7714	Li H,Shao YH,Wang Y,et al. Improving the precision of fluorescence lifetime measurement using a streak camera[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010005&dbname=CJFQ2010,2012, 2012.
APA	李恒,邵永红,王岩,屈军乐,&牛憨笨.(2012).Improving the precision of fluorescence lifetime measurement using a streak camera.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010005&dbname=CJFQ2010.
MLA	李恒,et al."Improving the precision of fluorescence lifetime measurement using a streak camera".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GXKB201010005&dbname=CJFQ2010 (2012).