Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor

CORC > 上海光学精密机械研究所 > 中国科学院上海光学精密机械研究所 > 精密光电测控研究与发展中心

	Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor
	Yan Zhongqiang ; Zhou Lei ; Zhao Yongkai ; Wang Jing ; Huang Lihua ; Hu Kongxin ; Liu Haihong ; Wang Hong ; Guo Zhaobiao ; Song Yajun ; Huang HJ(黄惠杰) ; Yang Ruifu
刊名	sens. actuator b-chem.
	2006
卷号	119 期号:2 页码:656
关键词	up-converting phosphor technology lateral-flow immunoassay sandwich immunoassay Yersinia pestis
ISSN号	0925-4005
中文摘要	up-converting phosphor technology (upt)-based lateral-flow immunoassay has been developed for quantitative detection of yersinia pestis rapidly and specifically. in this assay, 400 nm up-converting phosphor particles were used as the reporter. a sandwich immumoassay was employed by using a polyclonal antibody against f1 antigen of y. pestis immobilized on the nitrocellulose membrane and the same antibody conjugated to the upt particles. the signal detection of the strips was performed by the upt-based biosensor that could provide a 980 nm ir laser to excite the phosphor particles, then collect the visible luminescence emitted by the upt particles and finally convert it to the voltage as a signal. v-t and v-c stand for the multiplied voltage units for the test and the control line, respectively, and the ratio v-t/v-c is directly proportional to the number of y pestis in a sample. we observed a good linearity between the ratio and log cfu/ml of y pestis above the detection limit, which was approximately 10(4) cfu/mi. the precision of the intra- and inter-assay was below 15% (coefficient of variation, cv). cross-reactivity with related gram-negative enteric bacteria was not found. the upt-lf immunoassay system presented here takes less than 30 min to perform from the sample treatment to the data analysis. the current paper includes only preliminary data concerning the biomedical aspects of the assay, but is more concentrated on the technical details of establishing a rapid manual assay using a state-of-the-art label chemistry. (c) 2006 elsevier b.v. all rights reserved.
收录类别	EI
语种	英语
WOS记录号	WOS:000241197600044
公开日期	2009-09-24
内容类型	期刊论文
源URL	[http://ir.siom.ac.cn/handle/181231/6229]
专题	上海光学精密机械研究所_精密光电测控研究与发展中心
推荐引用方式 GB/T 7714	Yan Zhongqiang,Zhou Lei,Zhao Yongkai,et al. Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor[J]. sens. actuator b-chem.,2006,119(2):656, 663.
APA	Yan Zhongqiang.,Zhou Lei.,Zhao Yongkai.,Wang Jing.,Huang Lihua.,...&Yang Ruifu.(2006).Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor.sens. actuator b-chem.,119(2),656.
MLA	Yan Zhongqiang,et al."Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor".sens. actuator b-chem. 119.2(2006):656.