Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells

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	Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells
	Xu, Qingsong 1,2; Niu, Xueming 1; Wang, Wenjing 3; Yang, Wen 1; Du, Yuguang 4; Gu, Jianguo 1,2; Song, Linsheng 1
刊名	GLYCOCONJUGATE JOURNAL
	2017-04-01
卷号	34 期号:2 页码:219-227
关键词	Epithelial-mesenchymal transition Cell density N-glycan Migration Tumor
ISSN号	0282-0080
英文摘要	Epithelial-mesenchymal transition (EMT) is a process in tumor progression during which cancer cells undergo dramatic changes acquiring highly invasive properties. In a widespread adoption of TGF-beta-induced EMT model, we have previously observed that expression of bisecting GlcNAc on N-glycans was dramatically decreased. Herein, we performed in vitro studies with the MCF10A cell line. In response to low cell density, MCF10A cells suffered spontaneously morphologic and phenotypic EMT-like changes, including elongated spindle shape, extended out from edge of the cell sheet, cytoskeleton reorganization, vimentin and fibronectin up-regulation, catenins redistribution, and cadherin switching. Moreover, these phenotypic changes were associated with specific N-glycan alterations. Interestingly, the amounts of bisecting GlcNAc structure were declined, by contrast, the formation of beta 1-6 GlcNAc branches were obviously up-regulated during the EMT induced by sparse cell conditions. We further investigated N-glycans on the beta 1-integrin, which is a good target of some glycosyltransferases. The reactivity with E4-PHA lectin decreased, whereas the staining for L4-PHA lectin, which recognizes branched GlcNAc, increased in sparse cell conditions compared with dense cell conditions. Taken together, these results demonstrated that specific N-glycan alterations are coupled in EMT process and promoted cells migration at a low cell density.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	TO-MESENCHYMAL TRANSITION ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; BREAST-CANCER ; TUMOR MICROENVIRONMENT ; GLYCOSYLATION ; METASTASIS ; MIGRATION ; GROWTH ; SUPPRESSION ; PROGRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000396132800009
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/22136]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 2.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan 3.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xu, Qingsong,Niu, Xueming,Wang, Wenjing,et al. Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells[J]. GLYCOCONJUGATE JOURNAL,2017,34(2):219-227.
APA	Xu, Qingsong.,Niu, Xueming.,Wang, Wenjing.,Yang, Wen.,Du, Yuguang.,...&Song, Linsheng.(2017).Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells.GLYCOCONJUGATE JOURNAL,34(2),219-227.
MLA	Xu, Qingsong,et al."Specific N-glycan alterations are coupled in EMT induced by different density cultivation of MCF 10A epithelial cells".GLYCOCONJUGATE JOURNAL 34.2(2017):219-227.