Confinement Alters the Structure and Function of Calmodulin

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Confinement Alters the Structure and Function of Calmodulin
	Xu, Guohua 1; Cheng, Kai 1,2; Wu, Qiong 1; Liu, Maili 1; Li, Conggang 1
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2017-01-09
卷号	56 期号:2 页码:530-534
关键词	calmodulin confinement NMR spectroscopy protein structures reverse micelles
英文摘要	Many cellular reactions involving proteins, including their biosynthesis, misfolding, and transport, occur in confined compartments. Despite its importance, a structural basis of understanding of how confined environments alter protein function is still lacking. Herein, we explore structure-function correlations of calmodulin (CaM), a multidomain protein involved in many calcium-mediated signaling pathways, in reverse micelles. Confinement dramatically alters CaM structure and function. The protein forms an extended structure in bulk water, but becomes compacted in reverse micelles. In addition, confinement changes the function of CaM. Specifically, the protein binds the MLCK, AcN19, and somatostatin peptides in dilute buffer, but binds only the MLCK and AcN19 peptides in reverse micelles. In summary, we determined a new CaM structure in reverse micelles and demonstrate that confinement can modulate both protein structure and function.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	MOLECULAR-STRUCTURE DETERMINATION ; REVERSE MICELLES ; XPLOR-NIH ; ENCAPSULATED PROTEINS ; BACKBONE DYNAMICS ; NMR-SPECTROSCOPY ; PARAMAGNETIC NMR ; LOW-TEMPERATURE ; UBIQUITIN ; SPECTRA
收录类别	SCI
语种	英语
WOS记录号	WOS:000394996100013
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/10027]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Collaborat Inn, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Xu, Guohua,Cheng, Kai,Wu, Qiong,et al. Confinement Alters the Structure and Function of Calmodulin[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(2):530-534.
APA	Xu, Guohua,Cheng, Kai,Wu, Qiong,Liu, Maili,&Li, Conggang.(2017).Confinement Alters the Structure and Function of Calmodulin.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(2),530-534.
MLA	Xu, Guohua,et al."Confinement Alters the Structure and Function of Calmodulin".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.2(2017):530-534.