Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling

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	Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling
	Pan, Jia-Hong 1; Feng, Lin 1,2,3; Jiang, Wei-Dan 1,2,3; Wu, Pei 1,2,3; Kuang, Sheng-Yao 4; Tang, Ling 4; Zhang, Yong-An 5; Zhou, Xiao-Qiu 1,2,3; Liu, Yang 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017
卷号	60 页码:219-236
关键词	Vitamin E Grass carp (Ctenopharyngodon idella) Immunity Antioxidant capacity Apoptosis Immune organs
ISSN号	1050-4648
英文摘要	This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/kg diet) for 10 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. The results showed that compared with optimal vitamin E supplementation, vitamin E deficiency caused depressed growth, poor survival rates and increased skin lesion morbidity in grass carp. Meanwhile, vitamin E deficiency decreased lysozyme and acid phosphatase activities, complement component 3 and complement component 4 contents in the head kidney, spleen and skin of grass carp (P < 0.05). Moreover, vitamin E deficiency down-regulated antimicrobial peptides (Hepcidin, liver-expressed antimicrobial peptide-2A,-2B, beta-defensin), IL-10, TGF beta 1, I kappa B alpha, TOR and S6K1 mRNA levels (P < 0.05) and up-regulated IL-1 beta, IL-6, IL-8, IFN-gamma 2 and TNF alpha, NF-kappa B p65, IKK alpha, IKK beta and 4EBP1 (not in the head kidney) mRNA levels (P < 0.05). In addition, vitamin E deficiency caused oxidative damage, decreased superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione reductase (GR) activities, and down regulated the mRNA levels of antioxidant enzymes and signaling molecules Nrf2 (P < 0.05). Vitamin E deficiency also induced apoptosis by up-regulating capase-2,-3,-7, and-8 mRNA levels in the head kidney, spleen and skin of grass carp. In conclusion, this study indicated that dietary vitamin E deficiency depressed fish growth, impaired the immune function and disturbed the structural integrity of the head kidney, spleen and skin in grass carp, but optimal vitamin E supplementation can reverse those negative effects in fish. The optimal vitamin E requirements for young grass carp (266.39-1026.63 g) to achieve optimal growth performance and disease resistance based on the percent weight gain (PWG) and skin lesion morbidity were estimated to be 116.2 and 130.9 mg/kg diet, respectively. Meanwhile, based on immune indicator (LA activity in the head kidney) and antioxidant indicator (protection of spleen against MDA), the optimal vitamin E requirements for young grass carp were estimated to be 123.8 and 136.4 mg/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	TURBOT SCOPHTHALMUS-MAXIMUS ; PRO-INFLAMMATORY CYTOKINES ; SPLEEN FOLLOWING INFECTION ; METHIONINE HYDROXY ANALOG ; HEAD KIDNEY ; ANTIOXIDANT STATUS ; ALPHA-TOCOPHEROL ; CYPRINUS-CARPIO ; GENE-EXPRESSION ; JUVENILE JIAN
收录类别	SCI
语种	英语
WOS记录号	WOS:000392770000026
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28939]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ, Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Pan, Jia-Hong,Feng, Lin,Jiang, Wei-Dan,et al. Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:219-236.
APA	Pan, Jia-Hong.,Feng, Lin.,Jiang, Wei-Dan.,Wu, Pei.,Kuang, Sheng-Yao.,...&Liu, Yang.(2017).Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling.FISH & SHELLFISH IMMUNOLOGY,60,219-236.
MLA	Pan, Jia-Hong,et al."Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling".FISH & SHELLFISH IMMUNOLOGY 60(2017):219-236.