Production of Succinate from Acetate by Metabolically Engineered Escherichia coli

	Production of Succinate from Acetate by Metabolically Engineered Escherichia coli
	Li, Yunjie 1,4; Huang, Bing 1; Wu, Hui 1; Li, Zhimin 1,2; Ye, Qin 1; Zhang, Y-H Percival 3,4
刊名	ACS SYNTHETIC BIOLOGY
	2016-11-01
卷号	5 期号:11 页码:1299-1307
关键词	acetate succinate metabolic engineering Escherichia coli malic enzyme citrate synthase
英文摘要	Acetate, a major component of industrial biological wastewater and of lignocellulosic biomass hydrolysate, could potentially be a less costly alternative carbon source. Here we engineered Escherichia coli MG1655 strain for succinate production from acetate as the sole carbon source. Strategies of metabolic engineering included the blockage of the TCA cycle, redirection of the gluconeogenesis pathway, and enhancement of the glyoxylate shunt. The engineered strain MG03 featuring the deletion of genes: succinate dehydrogenase (sdhAB), isocitrate lyase regulator (iclR), and malic enzymes (maeB) accumulated 6.86 mM of succinate in 72 h. MG03(pTrc99a-gltA) overexpressing citrate synthase (g1tA) accumulated 16.45 mM of succinate and the yield reached 0.46 mol/mol, about 92% of the maximum theoretical yield. Resting-cell was adopted for the conversion of acetate to succinate, and the highest concentration of succinate achieved 61.7 mM.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ACID PRODUCTION ; CORYNEBACTERIUM-GLUTAMICUM ; ACETIC-ACID ; CARBON ; GENES ; FERMENTATIONS ; STRATEGY ; METHANOL ; STRAINS ; GLUCOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000388428100014
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2279]
专题	天津工业生物技术研究所_总体研究部_期刊论文
作者单位	1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China 2.Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China 3.Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West Seventh Ave, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Li, Yunjie,Huang, Bing,Wu, Hui,et al. Production of Succinate from Acetate by Metabolically Engineered Escherichia coli[J]. ACS SYNTHETIC BIOLOGY,2016,5(11):1299-1307.
APA	Li, Yunjie,Huang, Bing,Wu, Hui,Li, Zhimin,Ye, Qin,&Zhang, Y-H Percival.(2016).Production of Succinate from Acetate by Metabolically Engineered Escherichia coli.ACS SYNTHETIC BIOLOGY,5(11),1299-1307.
MLA	Li, Yunjie,et al."Production of Succinate from Acetate by Metabolically Engineered Escherichia coli".ACS SYNTHETIC BIOLOGY 5.11(2016):1299-1307.