A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320 | |
Dong, Huina1,2; Li, Sha1; Fang, Huan1,2; Xia, Miaomiao1,2; Zheng, Ping1,2; Zhang, Dawei1,2; Sun, Jibin1,2 | |
刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING |
2016-10-01 | |
卷号 | 39期号:10页码:1527-1537 |
关键词 | Vitamin B-12 Sinorhizobium meliloti Cobalamin Whole genome sequence Response surface methodology |
英文摘要 | Vitamin B-12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC-MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S. meliloti 320. Its whole genome information indicates that this strain has a complete VB12 synthetic pathway, which paves the way for further metabolic engineering studies. The optimal carbon and nitrogen sources are sucrose and corn steep liquor (CSL) plus peptone. The optimal combination of sucrose and CSL was obtained by response surface methodology as they are the most suitable carbon and nitrogen sources, respectively. This strain could produce 140 +/- 4.2 mg L-1 vitamin B-12 after incubating for 7 days in the optimal medium. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
关键词[WOS] | PSEUDOMONAS-DENITRIFICANS ; MICROBIAL-PRODUCTION ; B-12 ; REMOVAL ; LIGAND ; ACID ; DNA |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000382117800004 |
内容类型 | 期刊论文 |
源URL | [http://124.16.173.210/handle/834782/2923] |
专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
推荐引用方式 GB/T 7714 | Dong, Huina,Li, Sha,Fang, Huan,et al. A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(10):1527-1537. |
APA | Dong, Huina.,Li, Sha.,Fang, Huan.,Xia, Miaomiao.,Zheng, Ping.,...&Sun, Jibin.(2016).A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(10),1527-1537. |
MLA | Dong, Huina,et al."A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.10(2016):1527-1537. |
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