Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production

	Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production
	Tan, Zaigao 1,2; Chen, Jing 1,2; Zhang, Xueli 1,2
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2016-12-01
卷号	9 期号:11
关键词	Escherichia coli Pentose phosphate pathway (PPP) Succinate Reducing equivalent Ribosome binding site library (RBSL) Multivariate modular metabolic engineering (MMME)
英文摘要	Background: Succinate biosynthesis of Escherichia coli is reducing equivalent-dependent and the EMP pathway serves as the primary reducing equivalent source under anaerobic condition. Compared with EMP, pentose phosphate pathway (PPP) is reducing equivalent-conserving but suffers from low efficacy. In this study, the ribosome binding site library and modified multivariate modular metabolic engineering (MMME) approaches are employed to overcome the low efficacy of PPP and thus increase succinate production.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	FATTY-ACIDS PRODUCTION ; METABOLIC EVOLUTION ; CORYNEBACTERIUM-GLUTAMICUM ; CONSERVING PATHWAYS ; OPTIMIZATION ; EXPRESSION ; GLUCOSE ; GENE ; OVERPRODUCTION ; STRAINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000389380100002
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2286]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, 32 XiQiDao,Tianjin Airport Econ Pk, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Tan, Zaigao,Chen, Jing,Zhang, Xueli. Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production[J]. BIOTECHNOLOGY FOR BIOFUELS,2016,9(11).
APA	Tan, Zaigao,Chen, Jing,&Zhang, Xueli.(2016).Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production.BIOTECHNOLOGY FOR BIOFUELS,9(11).
MLA	Tan, Zaigao,et al."Systematic engineering of pentose phosphate pathway improves Escherichia coli succinate production".BIOTECHNOLOGY FOR BIOFUELS 9.11(2016).