Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges

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	Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges
	Zhang, Yi-Heng Percival 1,2,3
刊名	BIOTECHNOLOGY ADVANCES
	2015-11-15
卷号	33 期号:7 页码:1467-1483
关键词	Biochemicals Biofuels Economic analysis In vitro synthetic biosystem In vitro synthetic biology Innovative biomanufacturing Paradigm shift
英文摘要	The largest obstacle to the cost-competitive production of low-value and high-impact biofuels and biochemicals (called biocommodities) is high production costs catalyzed by microbes due to their inherent weaknesses, such as low product yield, slow reaction rate, high separation cost, intolerance to toxic products, and so on. This predominant whole-cell platform suffers from a mismatch between the primary goal of living microbes - cell proliferation and the desired biomanufacturing goal - desired products (not cell mass most times). In vitro synthetic biosystems consist of numerous enzymes as building bricks, enzyme complexes as building modules, and/or (biomimetic) coenzymes, which are assembled into synthetic enzymatic pathways for implementing complicated bioreactions. They emerge as an alternative solution for accomplishing a desired biotransformation without concerns of cell proliferation, complicated cellular regulation, and side-product formation. In addition to the most important advantage - high product yield, in vitro synthetic biosystems feature several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this perspective review, the general design rules of in vitro synthetic pathways are presented with eight supporting examples: hydrogen, n-butanol, isobutanol, electricity, starch, lactate,1,3-propanediol, and poly-3-hydroxylbutyrate. Also, a detailed economic analysis for enzymatic hydrogen production from carbohydrates is presented to illustrate some advantages of this system and the remaining challenges. Great market potentials will motivate worldwide efforts from multiple disciplines (i.e., chemistry, biology and engineering) to address the remaining obstacles pertaining to cost and stability of enzymes and coenzymes, standardized building parts and modules, biomimetic coenzymes, biosystem optimization, and scale-up, soon. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	BACTERIUM THERMOTOGA-MARITIMA ; CATALYZED ORGANIC-SYNTHESIS ; MOLECULAR-WEIGHT HEPARINS ; HIGH-YIELD PRODUCTION ; MICROBIAL FUEL-CELLS ; FATTY-ACID SYNTHASE ; ONE-POT SYNTHESIS ; ESCHERICHIA-COLI ; COFACTOR REGENERATION ; ENZYMATIC PATHWAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000366954200009
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2890]
专题	天津工业生物技术研究所_体外合成生物学研究组张以恒_期刊论文
作者单位	1.Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA 2.Cell Free Bioinnovat Inc, Blacksburg, VA 24060 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yi-Heng Percival. Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges[J]. BIOTECHNOLOGY ADVANCES,2015,33(7):1467-1483.
APA	Zhang, Yi-Heng Percival.(2015).Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges.BIOTECHNOLOGY ADVANCES,33(7),1467-1483.
MLA	Zhang, Yi-Heng Percival."Production of biofuels and biochemicals by in vitro synthetic biosystems: Opportunities and challenges".BIOTECHNOLOGY ADVANCES 33.7(2015):1467-1483.