Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii

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	Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii
	Cheng, Kun 1,2; Zhang, Fei 3; Sun, Fangfang 3; Chen, Hongge 1; Zhang, Y-H Percival 2,3,4
刊名	SCIENTIFIC REPORTS
	2015-08-20
卷号	5
英文摘要	Biobattery, a kind of enzymatic fuel cells, can convert organic compounds (e.g., glucose, starch) to electricity in a closed system without moving parts. Inspired by natural starch metabolism catalyzed by starch phosphorylase, isoamylase is essential to debranch alpha-1,6-glycosidic bonds of starch, yielding linear amylodextrin - the best fuel for sugar-powered biobattery. However, there is no thermostable isoamylase stable enough for simultaneous starch gelatinization and enzymatic hydrolysis, different from the case of thermostable alpha-amylase. A putative isoamylase gene was mined from megagenomic database. The open reading frame ST0928 from a hyperthermophilic archaeron Sulfolobus tokodaii was cloned and expressed in E. coli. The recombinant protein was easily purified by heat precipitation at 80 degrees C for 30 min. This enzyme was characterized and required Mg2+ as an activator. This enzyme was the most stable isoamylase reported with a half lifetime of 200 min at 90 degrees C in the presence of 0.5 mM MgCl2, suitable for simultaneous starch gelatinization and isoamylase hydrolysis. The cuvett-based air-breathing biobattery powered by isoamylase-treated starch exhibited nearly doubled power outputs than that powered by the same concentration starch solution, suggesting more glucose 1-phosphate generated.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	ESCHERICHIA-COLI ; SOLFATARICUS ATCC-35092 ; BIOFUEL CELLS ; GENE ; CLONING ; EXPRESSION ; PURIFICATION ; BACILLUS ; ENZYMES ; TRANSFORMATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000359770900001
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2886]
专题	天津工业生物技术研究所_体外合成生物学研究组张以恒_期刊论文
作者单位	1.Henan Agr Univ, Coll Life Sci, Zhengzhou 450002, Peoples R China 2.Virginia Tech, Biol Syst Engn Dept, Blacksburg, VA 24061 USA 3.Cell Free Bioinnovat Inc, Blacksburg, VA 24060 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Kun,Zhang, Fei,Sun, Fangfang,et al. Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii[J]. SCIENTIFIC REPORTS,2015,5.
APA	Cheng, Kun,Zhang, Fei,Sun, Fangfang,Chen, Hongge,&Zhang, Y-H Percival.(2015).Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii.SCIENTIFIC REPORTS,5.
MLA	Cheng, Kun,et al."Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii".SCIENTIFIC REPORTS 5(2015).