Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase
	Yan, Junjie 1; Liu, Weidong 2; Li, Yujie 2; Lai, Hui-Lin 3,4; Zheng, Yingying 2; Huang, Jian-Wen 3,4; Chen, Chun-Chi 2; Chen, Yun 5; Jin, Jian 5; Li, Huazhong 1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2016-06-17
卷号	475 期号:1 页码:8-12
关键词	1,4-beta-Endoglucanase Aspergillus niger Pichia pastoris Crystal structure
英文摘要	Eukaryotic 1,4-beta-endoglucanases (EC 3.2.1.4) have shown great potentials in many commercial applications because they effectively catalyze hydrolysis of cellulose, the main component of the plant cell wall. Here we expressed a glycoside hydrolase family (GH) 5 1,4-beta-endoglucanase from Aspergillus niger (AnCel5A) in Pichia pastoris, which exhibits outstanding pH and heat stability. In order to further investigate the molecular mechanism of AnCel5A, apo-form and cellotetraose (CH) complex enzyme crystal structures were solved to high resolution. AnCel5A folds into a typical (beta/alpha)(8)-TIM barrel architecture, resembling other GH5 members. In the substrate binding cavity, CTT is found to bind to -4--1 subsites, and several polyethylene glycol molecules are found in positive subsites. In addition, several unique N-glycosylation motifs that may contribute to protein higher stability were observed from crystal structures. These results are of great importance for understanding the molecular mechanism of AnCel5A, and also provide guidance for further applications of the enzyme. (C) 2016 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	THERMOASCUS-AURANTIACUS ; ENDOGLUCANASE ; PURIFICATION ; ACCURACY ; ANGSTROM ; ENZYMES ; CLONING ; CEL5A ; YEAST ; MODE
收录类别	SCI
语种	英语
WOS记录号	WOS:000377557800002
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2904]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Genozyme Biotechnol Inc, Taipei 106, Taiwan 4.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 5.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China
推荐引用方式 GB/T 7714	Yan, Junjie,Liu, Weidong,Li, Yujie,et al. Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,475(1):8-12.
APA	Yan, Junjie.,Liu, Weidong.,Li, Yujie.,Lai, Hui-Lin.,Zheng, Yingying.,...&Guo, Rey-Ting.(2016).Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,475(1),8-12.
MLA	Yan, Junjie,et al."Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 475.1(2016):8-12.