Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5

CORC > 天津工业生物技术研究所 > 结构生物学与蛋白酶学实验室郭瑞庭 > 期刊论文

	Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5
	Zhang, Lilan 1; Chen, Chun-Chi 1; Ko, Tzu-Ping 2; Huang, Jian-Wen 3; Zheng, Yingying 1; Liu, Weidong 1; Wang, Iren 2; Malwal, Satish R.4; Feng, Xinxin 4; Wang, Ke 4
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2016-04-04
卷号	55 期号:15 页码:4716-4720
关键词	biosynthesis drug discovery enzyme mechanisms isoprenoids protein structure
英文摘要	The structure of MoeN5, a unique prenyltransferase involved in the biosynthesis of the antibiotic moenomycin, is reported. MoeN5 catalyzes the reaction of geranyl diphosphate (GPP) with the cis-farnesyl group in phosphoglycolipid 5 to form the (C-25) moenocinyl-sidechain-containing lipid 7. GPP binds to an allylic site (S1) and aligns well with known S1 inhibitors. Alkyl glycosides, glycolipids, can bind to both S1 and a second site, S2. Long sidechains in S2 are bent and co-locate with the homoallylic substrate isopentenyl diphosphate in other prenyltransferases. These observations support a MoeN5 mechanism in which 5 binds to S2 with its C6-C11 group poised to attack C1 in GPP to form the moenocinyl sidechain, with the more distal regions of 5 aligning with the distal glucose in decyl maltoside. The results are of general interest because they provide the first structures of MoeN5 and a structural basis for its mechanism of action, results that will facilitate the design of new antibiotics.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	CELL WALL SYNTHESIS ; ANTIBIOTICS ; INHIBITORS ; SYNTHASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000373615900015
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2241]
专题	天津工业生物技术研究所_结构生物学与蛋白酶学实验室郭瑞庭_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 4.Univ Illinois, Dept Chem, 1209 W Calif St, Urbana, IL 61801 USA
推荐引用方式 GB/T 7714	Zhang, Lilan,Chen, Chun-Chi,Ko, Tzu-Ping,et al. Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016,55(15):4716-4720.
APA	Zhang, Lilan.,Chen, Chun-Chi.,Ko, Tzu-Ping.,Huang, Jian-Wen.,Zheng, Yingying.,...&Guo, Rey-Ting.(2016).Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,55(15),4716-4720.
MLA	Zhang, Lilan,et al."Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55.15(2016):4716-4720.