Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis

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	Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis
	Liu, Ying 1,2; Yan, Zhihui 2; Lu, Xiaoyun 2; Xiao, Dongguang 1; Jiang, Huifeng 2
刊名	SCIENTIFIC REPORTS
	2016-04-07
卷号	6
英文摘要	Protein rational design has become more and more popular for protein engineering with the advantage of biological big-data. In this study, we described a method of rational design that is able to identify desired mutants by analyzing the coevolution of protein sequence. We employed this approach to evolve an archaeal isopentenyl phosphate kinase that can convert dimethylallyl alcohol (DMA) into precursor of isoprenoids. By designing 9 point mutations, we improved the catalytic activities of IPK about 8-fold in vitro. After introducing the optimal mutant of IPK into engineered E. coli strain for beta-carotenoids production, we found that beta-carotenoids production exhibited 97% increase over the starting strain. The process of enzyme optimization presented here could be used to improve the catalytic activities of other enzymes.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	MEVALONATE-INDEPENDENT PATHWAY ; MULTIPLE SEQUENCE ALIGNMENT ; ESCHERICHIA-COLI ; ISOPRENOID BIOSYNTHESIS ; BETA-CAROTENE ; EVOLUTION ; RESIDUES ; DIPHOSPHATE ; ARCHAEA ; DESIGN
收录类别	SCI
语种	英语
WOS记录号	WOS:000373590200001
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2240]
专题	天津工业生物技术研究所_酵母基因组工程课题组江会锋_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ying,Yan, Zhihui,Lu, Xiaoyun,et al. Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, Ying,Yan, Zhihui,Lu, Xiaoyun,Xiao, Dongguang,&Jiang, Huifeng.(2016).Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis.SCIENTIFIC REPORTS,6.
MLA	Liu, Ying,et al."Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis".SCIENTIFIC REPORTS 6(2016).