Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 生物材料与组织工程研究中心 > 期刊论文

	Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages
	Chen, Zetao 1,2,3,4; Ni, Siyu 5; Han, Shengwei 3,4; Crawford, Ross 3,4; Lu, Shifeier 3,4; Wei, Fei 3,4; Chang, Jiang 6; Wu, Chengtie 6; Xiao, Yin 1,2,3,4
刊名	NANOSCALE
	2017
卷号	9 期号:2 页码:706-718
英文摘要	The osteoimmune environment plays indispensable roles in bone regeneration because the early immune environment that exists during the regenerative process promotes the recruitment and differentiation of osteoblastic lineage cells. The response of immune cells growing on nanotopographic surfaces and the microenvironment they generate should be considered when evaluating nanotopography-mediated osteogenesis, which are topics that are generally neglected in the field. In this study, we investigated the modulatory effects of nanoporous anodic alumina with different sized pores on macrophage responses and their subsequent effects on the osteogenic differentiation of bone marrow stromal cells (BMSCs). The nanopore structure and the pore size were found to be important adhesive cues for macrophages, which affected their spreading and cell shape, subsequently regulated the expression and activation of autophagy pathway components (LC3A/B, Beclin-1, Atg3, Atg7, and P62) and modulated the inflammatory response, osteoclastic activities, and release of osteogenic factors. Subsequently, the osteogenic pathways (Wnt and BMP) of BMSCs were found to be regulated by different nanopore-induced inflammatory environments, which affected the osteogenic differentiation outcomes. This study is the first to emphasize the effects of immune cells on nanotopography-mediated osteogenesis, which could lead to a new strategy for the development of advanced nanobiomaterials for tissue engineering, nanomedicine and immunotherapeutic applications.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]	MESENCHYMAL STEM-CELLS ; IN-VITRO ; IL-1-BETA PRODUCTION ; CARBON NANOTUBES ; ANODIC ALUMINA ; T-CELLS ; BONE ; AUTOPHAGY ; DIFFERENTIATION ; INFLAMMATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000394780200029
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23567]
专题	上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文
作者单位	1.Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Guangzhou 510055, Guangdong, Peoples R China 2.Guangdong Prov Key Lab Stomatol, Guangzhou 510055, Guangdong, Peoples R China 3.Queensland Univ Technol, Inst Hlth & Biomed Innovat, Brisbane, Qld 4059, Australia 4.Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Qld 4059, Australia 5.Donghua Univ Shanghai, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
推荐引用方式 GB/T 7714	Chen, Zetao,Ni, Siyu,Han, Shengwei,et al. Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages[J]. NANOSCALE,2017,9(2):706-718.
APA	Chen, Zetao.,Ni, Siyu.,Han, Shengwei.,Crawford, Ross.,Lu, Shifeier.,...&Xiao, Yin.(2017).Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages.NANOSCALE,9(2),706-718.
MLA	Chen, Zetao,et al."Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages".NANOSCALE 9.2(2017):706-718.