microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways
	Wei, Ran 1,2; Yang, Qin 1; Han, Bing 1,3; Li, Yan1 ; Yao, Kun 1; Yang, Xiuyu 1; Chen, Zexi 1,3; Yang, Shanshan 1; Zhou, Jiaqi 1; Li, Meizhang 1
刊名	ONCOTARGET
	2017
卷号	8 期号:10 页码:16633-16641
关键词	microRNA-375 colorectal cancer JAK2/STAT3 pathway MAP3K8/ERK pathway ATG7
英文摘要	MicroRNA-375 is involved in many types of alimentary system cancers. Our previous studies showed that microRNA-375 was significantly down-regulated in carcinoma tissues compared with para-carcinoma tissues, which strongly indicates that microRNA-375 might suppress the occurrence and development of colorectal cancer. However, the mechanism underlying the microRNA-375 regulation in colorectal cancer remains unclear. In this study, we first sorted out jak2, map3k8 and atg7 as microRNA-375 targeted genes from multiple databases, and found that jak2, map3k8 and their downstream genes stat3 and erk were up-regulated in carcinoma tissues. Secondly, we over-expressed microRNA-375 in colorectal cancer cell lines (HCT116, Caco2 and HT29). Our results showed that in microRNA-375 over-expressing cells, JAK2/STAT3 and MAP3K8/ERK proteins were down-regulated, cell proliferation was inhibited, cell migration rate did not change. There was no significant difference on ATG7 expression between the control group and microRNA-375 over-expressing HT29/ Caco2 cells, whereas microRNA-375 down-regulated ATG7 specifically in HCT116 cells. Finally, we demonstrated that expressing microRNA-375 suppressed tumor formation in nude mice. In conclusion, microRNA-375 might function as a tumor-repressive gene to inhibit cell proliferation, mainly through targeting both JAK2/STAT3 and MAP3K8/ERK signaling pathways in colorectal cancer. These findings suggest miR-375 as a promising diagnostic marker and a therapeutic drug for colorectal cancer.
类目[WOS]	Oncology ; Cell Biology
研究领域[WOS]	Oncology ; Cell Biology
关键词[WOS]	CARCINOMAS ; APOPTOSIS ; MAPK
收录类别	SCI
语种	英语
WOS记录号	WOS:000396024600045
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/46045]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 2.Yunnan Univ, Key Lab Tumor Mol Biol Yunnan Prov, Kunming 650091, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Wei, Ran,Yang, Qin,Han, Bing,et al. microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways[J]. ONCOTARGET,2017,8(10):16633-16641.
APA	Wei, Ran.,Yang, Qin.,Han, Bing.,Li, Yan.,Yao, Kun.,...&Cui, Qinghua.(2017).microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways.ONCOTARGET,8(10),16633-16641.
MLA	Wei, Ran,et al."microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways".ONCOTARGET 8.10(2017):16633-16641.