A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya | |
Li, Caiwen1,2; Chen, Tiantian1,2,3![]() | |
刊名 | HARMFUL ALGAE
![]() |
2017-02-01 | |
卷号 | 62页码:30-36 |
关键词 | Marine alveolate Parasite Life cycle Trophont Prevalence |
英文摘要 | The parasitic dinoflagellate Amoebophrya infects broad range of marine organisms. Particularly, Amoebophrya infections in planktonic dinoflagellates can prevent or delay the formation of algal blooms, and recycle undergrazed planktonic dinoflagellates back to the microbial loop by disrupting host cells. Its ecological significance was gradually recognized along with the discovery of its enormous molecular diversity in oceanic and coastal ecosystems. Thus, we developed a reliable, easily accessible and less time-consuming assay, to detect and assess Amoebophrya infections in planktonic dinoflagellates. The modified hematoxylin and eosin staining assay provided reliable diagnosis of Amoebophrya infection by identifying the characteristic "beehive" of the multinucleate trophonts. After staining, the typical multinucleate "beehive" is evidently distinguishable from the compact nuclei of uninfected host cells. The modified hematoxylin and eosin (H & E) staining assay is easy to use, that can be routinely performed within 3 h (up to 20 samples/batch) using general laboratory equipment, supplies and chemical reagents. The produced slides with agar-embedded dinoflagellate cells can be stored for several months or even years in a dry place without noticeable loss in quality of staining. With suitable calculation, the modified H & E assay can be applied to assess the prevalence of Amoebophrya infection in planktonic dinoflagellates. This efficient and powerful assay will facilitate the investigation on the ecological roles of Amoebophryidae in coastal and oceanic ecosystem. (C) 2016 Elsevier B.V. All rights reserved. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000393929800004 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://ir.qdio.ac.cn/handle/337002/136388] ![]() |
专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Caiwen,Chen, Tiantian. A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya[J]. HARMFUL ALGAE,2017,62:30-36. |
APA | Li, Caiwen,&Chen, Tiantian.(2017).A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya.HARMFUL ALGAE,62,30-36. |
MLA | Li, Caiwen,et al."A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya".HARMFUL ALGAE 62(2017):30-36. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论