滇池沉积物中好氧氨氧化细菌群落多样性研究 | |
陈泽斌1,2; 李冰3; 任禛1; 靳松1; 何峰5; 夏体渊[*]1; 王定康4; 余磊1 | |
刊名 | 西南农业学报
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2016 | |
卷号 | 29期号:3页码:689-694 |
关键词 | 滇池 沉积物 好氧氨氧化菌 氨单加氧酶基因 |
其他题名 | Community Diversity of Aerobic Ammonium-oxidizing Bacteria in Sediments of Dianchi Lake |
通讯作者 | xiatiyuan@sohu.com |
合作状况 | 其它 |
中文摘要 | 从滇池沉积物中提取好氧氨氧化细菌(aerobic ammonium-oxidizing bacteria,AOB)的DNA,通过构建硝化作用氨单加氧酶基因(amo A)文库并划分操作分类单元(OTU)的方法,揭示好氧氨氧化细菌群落结构多样性。获得了80个amo A基因克隆,利用Mothur软件将相似度不小于97%的序列归为1个OTU,共得到22个OTUs,其中OTU1、OTU2、OTU3单元最大,分别包含了27、12、9条序列。对22个OTUs的代表序列进行系统发育分析表明,其序列都属于变形杆菌的β-亚纲,分布于亚硝化螺菌属、亚硝化弧菌属、亚硝化单胞菌属、亚硝化叶菌属4个属;优势种群为Nitrosomonas europaea的近缘种,尚未被描述过。 |
英文摘要 | The clone libraries of ammonium monooxygenase encoding gene (amoA) in the sediments of Dianchi lake were constructed and grouped into the operational taxonomic units (OTUs) to determine the community diversity of aerobic ammonium-oxidizing bacteria (AOB). A total of 80 sequences were obtained from Dianchi lake. 22 OTUs from Dianchi lake were grouped respectively based on a 3 % amoA gene sequence distance cutoff with the Mothur program. The largest groups were OTU1, OTU2 and OTU3 which consisted of 27, 12, 9 sequences respectively. Representative sequences of 22 OTUs were determined and a phylogenie tree was constructed. Phylogenetie analysis with the aligned sequences showed that the amoA sequences from Dianchi lake were all in the β-subdivision of Proteobacteria. These sequences belonged to genus Nitrosovibrio, Nitrosospira, Nitrosomonas and Nitrosolobus. The dominated β-AOB populations was the closely related species of Nitrosomonas europaea which had not been described. |
收录类别 | 其他 |
资助信息 | 国家自然科学基金项目(31460491);云南省科技厅应用基础研究计划青年项目(2013FD040);云南省教育厅科学研究项目(2014Y390);昆明学院人才引进项目(YJL14005);昆明学院人才引进项目(YJL12010);昆明学院科学研究项目(XJL13018);云南中烟品牌省内原料产地生态与主栽品种合理布局研究项目;云南省高校优势特色重点学科(生态学)建设项目资助 |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.26:8080/handle/152453/9637] ![]() |
专题 | 昆明动物研究所_中国科学院昆明灵长类研究中心 |
作者单位 | 1.昆明学院农学院;昆明650214 2.云南省都市特色农业工程技术研究中心;昆明650214 3.中国科学院昆明动物研究所;昆明650223 4.昆明学院生命科学与技术系;昆明650214 5.昆明市滇池生态研究所;昆明650228 |
推荐引用方式 GB/T 7714 | 陈泽斌,李冰,任禛,等. 滇池沉积物中好氧氨氧化细菌群落多样性研究[J]. 西南农业学报,2016,29(3):689-694. |
APA | 陈泽斌.,李冰.,任禛.,靳松.,何峰.,...&余磊.(2016).滇池沉积物中好氧氨氧化细菌群落多样性研究.西南农业学报,29(3),689-694. |
MLA | 陈泽斌,et al."滇池沉积物中好氧氨氧化细菌群落多样性研究".西南农业学报 29.3(2016):689-694. |
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