Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 生物材料与组织工程研究中心 > 期刊论文

	Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment
	Zhang, Lei 1; Xiong, Zhichao 2; Chen, Yajing 1; Peng, Li 1; Yu, Bohao 1; Gao, Xiaodi 1; Zhang, Runsheng 3; Zhang, Lingyi 1; Zhang, Weibing 1
刊名	RSC ADVANCES
	2016
卷号	6 期号:36 页码:30014-30020
英文摘要	Novel nanostructure metallic zirconia incorporated ordered mesoporous carbon (OMC) composites were synthesized via a multi-component co-assembly strategy associated with a direct carbonization process from resol, ZrO(NO3)(2)center dot xH(2)O, and triblock copolymer F127. Herein, the novel metallic zirconia incorporated OMC as a Metal Oxide Affinity Chromatography (MOAC) material had large mesopores (5.6 nm), a high surface area (387 m(2) g(-1)), a large pore volume (0.35 cm(3) g(-1)) and excellent hydrophilicity for the selective enrichment of phosphopeptides. The surface construction and chemical composition of the material were characterized by SEM, TEM, XPS, EDS, BET, and XRD. Experimental conditions for trapping phosphopeptides were optimized using beta-casein as the standard protein. Under the optimal conditions combined with the merits of high surface area of material and special combination with phosphopeptides, the zirconia/OMC composites for enrichment of the phosphopeptides showed a high selectivity (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 300) and lower detection sensitivity (1.5 fmol). Moreover, the as-prepared MOAC zirconia/OMC composites were successfully applied to the detection and identification of low-abundance phosphopeptides from non-fat milk.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	SELECTIVE ENRICHMENT ; FACILE SYNTHESIS ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN-PHOSPHORYLATION ; MAGNETIC NANOPARTICLES ; NANOCRYSTAL CLUSTERS ; CAPTURE ; SHELL ; MICROSPHERES ; SAMPLE
收录类别	SCI
语种	英语
WOS记录号	WOS:000373061600017
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23007]
专题	上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文
作者单位	1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China 3.Shanghai Publ Secur Bur, Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lei,Xiong, Zhichao,Chen, Yajing,et al. Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment[J]. RSC ADVANCES,2016,6(36):30014-30020.
APA	Zhang, Lei.,Xiong, Zhichao.,Chen, Yajing.,Peng, Li.,Yu, Bohao.,...&Zhang, Weibing.(2016).Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment.RSC ADVANCES,6(36),30014-30020.
MLA	Zhang, Lei,et al."Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment".RSC ADVANCES 6.36(2016):30014-30020.