Upconversion fluorescence resonance energy transfer-a novel approach for sensitive detection of fluoroquinolones in water samples

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Upconversion fluorescence resonance energy transfer-a novel approach for sensitive detection of fluoroquinolones in water samples
	Zhang, Zhen; Zhang, Meng; Wu, Xiang-yang; Chang, Zhen; Lee, Yong-Iii; Huy, Bui The; Sakthivel, Kavitha; Liu, Jing-fu; Jiang, Gui-bin
刊名	MICROCHEMICAL JOURNAL
	2016-01
卷号	124 期号:0 页码:181-187
关键词	Upconversion Fluoroquinolone Fluorescence resonance energy transfer Nanoparticle
英文摘要	A novel homogeneous assay method was put forth for simultaneous sensing of fluoroquinolone derivatives (FQs) in water utilizing the upconversion fluorescence resonance energy transfer (FRET) process based on the competitive reaction between dissolved FQs and labeled AuNPs for FQs Mab connected UCPs. The prepared size-tunable beta-NaLuF4:Yb,Er,Gd upconversion phosphors (UCPs) were functionalized with carboxylic acid (COOH-) and monoclonal antibody (Mab, C2F3C2) to act as a donor. The gold nanoparticles (AuNPs) labeled with the corresponding antigen (ciprofloxacin-BSA) take the role of an acceptor. Under optimized conditions, the limit of detections (LODs) for three common FQs, enrofloxacin (ENR), ciprofloxaxin (CIP), and norfloxacin (NOR), were 0.19-032 ng/mL based on 3 sigma. The recoveries were found to be in the range of 73.5-114.5% (normalized value according to the cross-reactivity of Mab) for water samples (tap water, pond water and river water). The proposed approach possesses significant advantages as follows: (i) simple procedure (only one step) without washing and separation steps, and no sample pretreatment other than filtration; (ii) fast liquid-phase kinetics with shortened incubation time; and (iii) high tolerance to various interfering substances. All of which indicated its potentiality as an efficient biosensor towards the monitoring of FQs in aquatic environments. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35977]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Zhen,Zhang, Meng,Wu, Xiang-yang,et al. Upconversion fluorescence resonance energy transfer-a novel approach for sensitive detection of fluoroquinolones in water samples[J]. MICROCHEMICAL JOURNAL,2016,124(0):181-187.
APA	Zhang, Zhen.,Zhang, Meng.,Wu, Xiang-yang.,Chang, Zhen.,Lee, Yong-Iii.,...&Jiang, Gui-bin.(2016).Upconversion fluorescence resonance energy transfer-a novel approach for sensitive detection of fluoroquinolones in water samples.MICROCHEMICAL JOURNAL,124(0),181-187.
MLA	Zhang, Zhen,et al."Upconversion fluorescence resonance energy transfer-a novel approach for sensitive detection of fluoroquinolones in water samples".MICROCHEMICAL JOURNAL 124.0(2016):181-187.