The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy | |
Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超) | |
刊名 | Science China Materials |
2017 | |
卷号 | 60期号:3页码:269-278 |
关键词 | AFM plasmid 化疗药物 DNA methotrexate arrows 甲氨蝶呤 length 原子力显微镜 purine |
ISSN号 | 2095-8226 |
其他题名 | 基于AFM的化疗药物与质粒DNA间动态相互作用观测 |
通讯作者 | 刘连庆 ; 席宁 |
产权排序 | 1 |
中文摘要 | The advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level. |
WOS标题词 | Science & Technology ; Technology |
类目[WOS] | Materials Science, Multidisciplinary |
研究领域[WOS] | Materials Science |
关键词[WOS] | SINGLE-MOLECULE ; CANCER-CELLS ; MEASUREMENTS REVEAL ; PERSISTENCE LENGTH ; MEMBRANE-PROTEINS ; SPECTROSCOPY ; CISPLATIN ; TEMPERATURE ; DEPENDENCE ; MORPHOLOGY |
收录类别 | SCI ; CSCD |
语种 | 英语 |
WOS记录号 | WOS:000398129000008 |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/20246] |
专题 | 沈阳自动化研究所_机器人学研究室 |
推荐引用方式 GB/T 7714 | Li M,Liu LQ,Xiao XB,et al. The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy[J]. Science China Materials,2017,60(3):269-278. |
APA | Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2017).The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy.Science China Materials,60(3),269-278. |
MLA | Li M,et al."The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy".Science China Materials 60.3(2017):269-278. |
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